KIAA1592

Product ID	ORK00252
Accession No	AB046812
Description	cyclin and CBS domain divalent metal cation transport mediator 4
Clone name	fj09188
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4510 bp) Predicted protein sequence (717 aa)
HaloTag ORF Clone	FHC00252
Flexi ORF Clone	FXC00252
Source	Human fetal brain
Rouge ID	mKIAA1592 by Kazusa Mouse cDNA Project

Length: 4510 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2354 bp
Genome contig ID	gi89161199f_96690636
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TATTTTTGATTGGTAATAAAAAATCAAATATGTAT
Flanking genome sequence (150701 - 150750)	----+--------+--------+--------+--------+----* AAATCCTGGTGAATCTACAACTTGCCTGTTTGTTCTGTCAGTATTCAGTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	f	96790636	96841335	7	100.0	Perfect prediction

Length: 717 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW71340	100.0	cyclin M4 [Homo...	Homo sapiens
Q6P4Q7	99.9	Metal transport...	Homo sapiens
XP_538467	93.3	similar to cycl...	Canis lupus fam...
XP_001916387	92.6	similar to Meta...	Equus caballus
P0C588	91.1	Metal transport...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002550	126	300	PF01595	Protein of unknown function DUF21
HMMPfam	IPR000644	339	446	PF00571	Cystathionine beta-synthase
ProfileScan	IPR000595	517	594	PS50042	Cyclic nucleotide-binding

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	127	HILLITVLLVLSGIFSGLNLGLM	149	PRIMARY	23
	2	182	NYLLCSLLLGNVLVNTSLTILLD	204	SECONDARY	23
	3	212	MAVASSTIGIVIFGEILPQALCS	234	SECONDARY	23
	4	243	NTILLTKFFMLLTFPLSFPISKL	265	SECONDARY	23

Primer_f	ACATCAAGATCACTCGGCAGC
Primer_r	TCGTTGAGAAGAGTTGTGGTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-