|
Order Kazusa clone(s) from : |
| Product ID | ORK05679 |
|---|---|
| Accession No | AB046822 |
| Description | NCK-associated protein 5-like |
| Clone name | fj10252 |
| Vector information | |
| cDNA sequence | DNA sequence (4235 bp) Predicted protein sequence (1003 aa) |
| Source | Human fetal brain |
Length: 4235 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | Warning |
Integrity of 3' end
| Length of 3'UTR | 1222 bp |
|---|---|
| Genome contig ID | gi89161190r_48371196 |
| PolyA signal sequence (AATAAA,-26) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 12 | r | 48471196 | 48477052 | 5 | 100.0 | Perfect prediction |
Length: 1003 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)
RT-PCR-ELISA
|
Experimental conditions| Primer_f | CTCCCCTTCCTTAGCATGTTC |
|---|---|
| Primer_r | AAACTTGAGACCCCTAGCTGG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 12
Experimental conditions| Panel name | RH-map |
|---|---|
| Primer_f | CTCCCCTTCCTTAGCATGTTC |
| Primer_r | AAACTTGAGACCCCTAGCTGG |
| PCR product length | 168 bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |