KIAA1889

Product ID	ORK07376
Accession No	AB067476
Description	XK, Kell blood group complex subunit-related family, member 4
Clone name	fk02634
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3441 bp) Predicted protein sequence (505 aa)
Source	Human fetal brain
Rouge ID	mKIAA1889 by Kazusa Mouse cDNA Project

Length: 3441 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1922 bp
Genome contig ID	gi51511724f_56078037
PolyA signal sequence (None)	+--------+--------+----*----+---- ACCCAACTGATAGATTAAGTATTAGTCAAACCACT
Flanking genome sequence (523227 - 523276)	----+--------+--------+--------+--------+----* AAAAAAGAAAAAGAAAAAAGTTTAACTTAATTATTCGGTTATTTGGATCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	f	56178037	56601262	3	100.0	Perfect prediction

Length: 505 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q5GH76	1.9e-201	100.0	XK-related prot...	Homo sapiens
Q49LS4	8e-201	99.6	XK-related prot...	Pan troglodytes
XP_851429	9e-196	97.0	similar to XK-r...	Canis lupus fam...
Q5GH59	8.1e-194	95.8	XK-related prot...	Rattus norvegicus
Q5GH67	2.9e-193	95.8	XK-related prot...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	GLTLFFVVLGSLSVQVFSFRWFV	23	SECONDARY	23
	2	180	IVQTHSLQALQGFTAAASLVSLA	202	SECONDARY	23
	3	223	SYMAVIIQFCWHFFTIAARVITF	245	PRIMARY	23
	4	252	FQLYFGIFIVLHWCIMTFWIVHC	274	PRIMARY	23
	5	283	WEEIVFDMVVGIIYIFSWFNVK	304	PRIMARY	22
	6	311	RLFIYYFVILLENTALSALWYLY	333	PRIMARY	23
	7	342	FAIPALCVVFSSFLTGVVFMLMY	364	PRIMARY	23

Primer_f	CCATGTGTGAAAATCCTATCC
Primer_r	TCTGTCAAAAAGGATGTTCGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-