KIAA1968

Gene/Protein Characteristic Table for KIAA1968

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Product ID	ORK04075
Accession No	AB075848
Description	AT-hook transcription factor
Clone name	fk04713
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3334 bp) Predicted protein sequence (860 aa)
Source	Human fetal brain
Rouge ID	mKIAA1968 by Kazusa Mouse cDNA Project

Features of the cloned cDNA sequence

Description

Length: 3334 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	503 bp
Genome contig ID	gi89161216r_116061470
PolyA signal sequence (None)	+--------+--------+----*----+---- ACTCCAGCCTGGGTGATAGTGTGAGACCCTGTCTC
Flanking genome sequence (99718 - 99669)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAGAAAAAAGAATATCATTCTCTTTTTCTGTCTCTGACC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	116161188	116190182	11	99.7	Perfect prediction

Features of the protein sequence

Description

Length: 860 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAC85132	99.9	FLJ00281 protei...	Homo sapiens
BAD18725	99.8	FLJ00356 protei...	Homo sapiens
CAI16863	99.9	AT-hook transcr...	Homo sapiens
AAH55285	99.9	AT-hook transcr...	Homo sapiens
Q7Z591	99.9	AT-hook-contain...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Expression profile

Description

RT-PCR-ELISA

Experimental conditions

Primer_f	CAAGTCCAGAAGCCACAACAG
Primer_r	CGTCTGAAATTCTTGGGGTAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information

Description

Chromosome No. 9

Experimental conditions

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-

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