KIAA1913

Product ID	ORK00298
Accession No	AB067500
Description	transmembrane protein 200A, transcript variant 4
Clone name	fk05496
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3512 bp) Predicted protein sequence (509 aa)
HaloTag ORF Clone	FHC00298
Flexi ORF Clone	FXC00298
Source	Human fetal brain
Rouge ID	mKIAA1913 by Kazusa Mouse cDNA Project

Length: 3512 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1165 bp
Genome contig ID	gi89161210f_130628572
PolyA signal sequence (AATAAA,-30)	+--------+--------+----*----+---- AAGATAATAAATATTAAAATGGATGTTTTCATCAG
Flanking genome sequence (177331 - 177380)	----+--------+--------+--------+--------+----* AAAATTTTCATGTTTTCCTTTAAGGTAACATAATTGTAAGAATTGTTTAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	f	130728572	130805901	3	100.0	Perfect prediction

Length: 509 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001504320	5.3e-188	94.5	transmembrane p...	Equus caballus
XP_589121	2.2e-186	93.7	similar to tran...	Bos taurus
XP_001927918	7.4e-186	92.7	transmembrane p...	Sus scrofa
Q8C817	1.5e-182	90.6	Transmembrane p...	Mus musculus
BAC27929	4.9e-182	90.4	unnamed protein...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	15	KGQAMIATGGVITGLAAL	32	SECONDARY	18
SOSUI2	2	74	RLYSPSGFFLILGVLISIIGIAM	96	PRIMARY	23

Primer_f	GTTTGGGTTACTTGTGACTGC
Primer_r	GTACTCCTGGTCCACACTTTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-