KIAA1815

Product ID	ORK04912
Accession No	AB058718
Description	endoplasmic reticulum metallopeptidase 1
Clone name	ha00534
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5214 bp) Predicted protein sequence (881 aa)
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA1815 by Kazusa Mouse cDNA Project
Note	We replaced pj01758, former representative clones for KIAA1815 with ha00534. (2002/12/27)

Length: 5214 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2568 bp
Genome contig ID	gi89161216r_5674573
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- GCCTTACAATGTTACTGAAATAAACTGTTTAATAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTGGTTGGTATCTTTTTAACTGAAAAACAGATCTGTGTTCTTCTGTATC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	5774573	5822958	15	99.5	Perfect prediction

Length: 881 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q7Z2K6	100.0	Endoplasmic ret...	Homo sapiens
XP_001924252	90.8	endoplasmic ret...	Sus scrofa
XP_001493226	91.8	endoplasmic ret...	Equus caballus
Q6UPR8	86.0	Endoplasmic ret...	Rattus norvegicus
Q3UVK0	85.5	Endoplasmic ret...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR007484	175	346	PF04389	Peptidase M28

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	50	AALGLALYLIALRTLVQLSLQQL	72	PRIMARY	23
	2	374	GNMVFFDVLGLFVIAY	389	PRIMARY	16
	3	395	SIINYMVVMGVVLYLG	410	PRIMARY	16
	4	432	GITLISWFTSLVTVLIIAVFISL	454	PRIMARY	23
	5	466	YVSVCLYGTATVAKIILIHTLA	487	PRIMARY	22
	6	498	QYLGEVFFDISLFVHCCFLVTLT	520	PRIMARY	23
	7	524	LCSAFISAVWVAFPLLTKLCVHK	546	PRIMARY	23
	8	559	AFYLLGMFIPYLYALYLIWAVFE	581	PRIMARY	23
	9	596	PDVVLASILAGCTMILSSYFINF	618	SECONDARY	23
	10	628	TMLTLTLVCAITFLLVCSGTFFP	650	PRIMARY	23

Primer_f	TTCCCTGTGTAAACCATTGTC
Primer_r	CAAACGGTATGCTTAACAGAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-