Gene/Protein Characteristic Table for KIAA0024
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Product ID ORK00370
Accession No D14694
Description phosphatidylserine synthase 1, transcript variant 1
Clone name ha00556
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (2504 bp)
Predicted protein sequence (507 aa)
Flexi ORF Clone FXC00370
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0024 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 2504 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning Warning
Integrity of 3' end
Length of 3'UTR 980 bp
Genome contig ID gi51511724f_97243343
PolyA signal sequence
(AATAAA,-26)
+----*----+----*----+----*----+----
CCTGTTTTAAATAAAAACGATTCACTTTAAAGCCT
Flanking genome sequence
(172609 - 172658)
----+----*----+----*----+----*----+----*----+----*
ATCAAAGGTCTGTCTGTAAAATGCTCATTTTCTTGCATCTTACTGGTATC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 8 f 97343343 97415950 13 99.6 Perfect prediction
Features of the protein sequence
Description

Length: 507 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001091545 5.5e-215 99.8 phosphatidylser...
Macaca mulatta
BAF85725 8.8e-215 99.8 unnamed protein...
Homo sapiens
Q99LH2 7.1e-212 97.3 Phosphatidylser...
Mus musculus
AAH87129 7.4e-211 97.0 Phosphatidylser...
Rattus norvegicus
AAI12834 1e-210 97.7 Phosphatidylser...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR004277 130 408 PF03034 Phosphatidyl serine synthase

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 1 LCRPAISLLWLVTPGVPAFS 20 SECONDARY 20
2 74 PHTITLLSFTIVSLMYFAFTRD 95 SECONDARY 22
3 103 IWRGILSVIFFFLIISVLAFPN 124 PRIMARY 22
4 135 WRMVFGLSVLYFLFLVFLLFLNF 157 PRIMARY 23
5 208 WGWAMKALLIRSYGLCWTISITW 230 SECONDARY 23
6 244 FAECWWDQVILDILLCNGGGIWL 266 SECONDARY 23
7 319 QRVAGVYLFMIIWQLTELNTFFL 341 SECONDARY 23
8 352 PLSWGRILFIGGITAPTVRQYY 373 SECONDARY 22
9 385 GTQCWVFGVIGFLEAIVCIKFGQ 407 PRIMARY 23
10 415 ILYVVLWLLCVAFTTFLCLYGMI 437 PRIMARY 23
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 8
Experimental conditions
Panel name Genebridge 4
Primer_f CAATCAGGAGAGGGTAGCTG
Primer_r CACCATGGAAGGAGAACAAA
PCR product length 178 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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