KIAA0024

Product ID	ORK00370
Accession No	D14694
Description	phosphatidylserine synthase 1, transcript variant 1
Clone name	ha00556
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2504 bp) Predicted protein sequence (507 aa)
HaloTag ORF Clone	FHC00370
Flexi ORF Clone	FXC00370
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0024 by Kazusa Mouse cDNA Project

Length: 2504 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	980 bp
Genome contig ID	gi51511724f_97243343
PolyA signal sequence (AATAAA,-26)	+--------+--------+----*----+---- CCTGTTTTAAATAAAAACGATTCACTTTAAAGCCT
Flanking genome sequence (172609 - 172658)	----+--------+--------+--------+--------+----* ATCAAAGGTCTGTCTGTAAAATGCTCATTTTCTTGCATCTTACTGGTATC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	f	97343343	97415950	13	99.6	Perfect prediction

Length: 507 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001091545	5.5e-215	99.8	phosphatidylser...	Macaca mulatta
BAF85725	8.8e-215	99.8	unnamed protein...	Homo sapiens
Q99LH2	7.1e-212	97.3	Phosphatidylser...	Mus musculus
AAH87129	7.4e-211	97.0	Phosphatidylser...	Rattus norvegicus
AAI12834	1e-210	97.7	Phosphatidylser...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004277	130	408	PF03034	Phosphatidyl serine synthase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	LCRPAISLLWLVTPGVPAFS	20	SECONDARY	20
	2	74	PHTITLLSFTIVSLMYFAFTRD	95	SECONDARY	22
	3	103	IWRGILSVIFFFLIISVLAFPN	124	PRIMARY	22
	4	135	WRMVFGLSVLYFLFLVFLLFLNF	157	PRIMARY	23
	5	208	WGWAMKALLIRSYGLCWTISITW	230	SECONDARY	23
	6	244	FAECWWDQVILDILLCNGGGIWL	266	SECONDARY	23
	7	319	QRVAGVYLFMIIWQLTELNTFFL	341	SECONDARY	23
	8	352	PLSWGRILFIGGITAPTVRQYY	373	SECONDARY	22
	9	385	GTQCWVFGVIGFLEAIVCIKFGQ	407	PRIMARY	23
	10	415	ILYVVLWLLCVAFTTFLCLYGMI	437	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	CAATCAGGAGAGGGTAGCTG
Primer_r	CACCATGGAAGGAGAACAAA
PCR product length	178 bp
PCR conditions	95 °C15 sec60 °C60 sec30 cycles