KIAA0102

Product ID	ORK00409
Accession No	D14658
Description	signal peptidase complex subunit 2
Clone name	ha00791
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (1370 bp) Predicted protein sequence (225 aa)
HaloTag ORF Clone	FHC00409
Flexi ORF Clone	FXC00409
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0102 by Kazusa Mouse cDNA Project

Length: 1370 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	691 bp
Genome contig ID	gi51511727f_74237981
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- CTAAAATTTTTTTCAATAAAAGGAAGGAAGATGTG
Flanking genome sequence (128448 - 128497)	----+--------+--------+--------+--------+----* AAAAAAATGGAGTCATAGTTTTGATGGAGAGAACAGAGAAACAAGTGTTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	f	74337981	74366427	5	100.0	Perfect prediction
Ensembl gnome browser	1	r	28294148	28295518	1	99.0	Perfect prediction

Length: 225 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q15005	7.7e-90	100.0	Signal peptidas...	Homo sapiens
BAG56680	1.4e-89	99.6	unnamed protein...	Homo sapiens
AAH64957	1.6e-89	99.6	SPCS2 protein [...	Homo sapiens
XP_001174940	1.9e-89	99.6	hypothetical pr...	Pan troglodytes
EAX07712	1.8e-88	99.1	hCG18930 [Homo ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR009582	42	223	PF06703	Microsomal signal peptidase 25 kDa subunit

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	82	GRLTICTISCFFAIVALIWDYMH	104	PRIMARY	23
SOSUI2	2	112	VLALCVISYFVMMGILTIYTSY	133	SECONDARY	22

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	AGTGGGAGAACAAAGCAGCAG
Primer_r	TTTTTATCTCCCCCACCCCCC
PCR product length	223 bp
PCR conditions	95 °C15 sec70 °C60 sec32 cycles