KIAA0062

Product ID	ORK00389
Accession No	D31887
Description	solute carrier family 39 (zinc transporter), member 14, transcript variant 1
Clone name	ha01020
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4573 bp) Predicted protein sequence (531 aa)
HaloTag ORF Clone	FHC00389
Flexi ORF Clone	FXC00389
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0062 by Kazusa Mouse cDNA Project

Length: 4573 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2975 bp
Genome contig ID	gi51511724f_22180763
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- ATTTGTAAGATTCTATAAATAAAGCTATATTCTGT
Flanking genome sequence (155370 - 155419)	----+--------+--------+--------+--------+----* AATTGTTGAGAATCCCACGGGTGATCATTTGCAATAAATGTGGATGTAAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	f	22280763	22336131	9	99.2	Perfect prediction

Length: 531 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
CAH91293	0	97.9	hypothetical pr...	Pongo abelii
XP_001106660	1e-209	93.2	similar to solu...	Macaca mulatta
EAW63682	1.2e-209	100.0	solute carrier ...	Homo sapiens
BAG58625	4.7e-209	99.8	unnamed protein...	Homo sapiens
Q15043	4.7e-209	99.8	Zinc transporte...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033091	1.3e-30	31.2	KIAA1265

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003689	190	522	PF02535	Zinc/iron permease

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	190	VEVWGYGLLCVTVISLCSLLGAS	212	PRIMARY	23
	2	225	LLLYFIALAIGTLYSNALFQLIP	247	SECONDARY	23
	3	262	KSAVVFGGFYLFFFTEKILKILL	284	SECONDARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	TGTCAGGATGCTCACTTGTTC
Primer_r	CAGTGGGGAAGAGGAGGTCAA
PCR product length	207 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles