KIAA0033

Product ID	ORK00377
Accession No	D26067
Description	transmembrane protein 41B, transcript variant 1
Clone name	ha02009
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3269 bp) Predicted protein sequence (335 aa)
HaloTag ORF Clone	FHC00377
Flexi ORF Clone	FXC00377
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0033 by Kazusa Mouse cDNA Project

Length: 3269 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2260 bp
Genome contig ID	gi51511727r_9159292
PolyA signal sequence (AATAAA,-30)	+--------+--------+----*----+---- TTAAAAATAAAAGAGCTTAGACTCAGTAGGAACTC
Flanking genome sequence (99995 - 99946)	----+--------+--------+--------+--------+----* AGTAGAAGCTTCACTATTTACTCCAGCGTGTGTAAATTGTACTTACTCTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	r	9259287	9292697	7	99.3	Perfect prediction

Length: 335 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q5BJD5	1.9e-114	100.0	Transmembrane p...	Homo sapiens
BAG64402	7.3e-114	99.7	unnamed protein...	Homo sapiens
Q5RBZ8	3.3e-111	97.6	Transmembrane p...	Pongo abelii
XP_001504961	8.5e-107	94.2	similar to MGC1...	Equus caballus
XP_851421	5.1e-106	93.8	similar to tran...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR015414	173	294	PF09335	SNARE associated Golgi protein
ScanRegExp	IPR002114	302	317	PS00589	Phosphotransferase system

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	VVSGEEVAPEVALAVAL	17	SECONDARY	17
	2	96	MSLLILVSIFLSAAFVMFLVYKN	118	PRIMARY	23
	3	157	VLVAYFATYIFLQTFAIPGSIFL	179	PRIMARY	23
	4	191	LALFLVCLCSGLGASFCYMLSYL	213	PRIMARY	23
	5	270	LKVFFIGTFLGVAPPSFVAIKAG	292	SECONDARY	23
	6	306	SWNSIFILMILAVLSILPAIFQ	327	PRIMARY	22

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	TAATCATCCTCAAAGCCTGTT
Primer_r	TAAATAATCCTCACGCAAAAG
PCR product length	155 bp
PCR conditions	95 °C15 sec60 °C60 sec30 cycles