KIAA0206

Product ID	ORK05824
Accession No	D86961
Description	lipoma HMGIC fusion partner-like 2
Clone name	ha04806
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4249 bp) Predicted protein sequence (193 aa)
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0206 by Kazusa Mouse cDNA Project

Length: 4249 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3667 bp
Genome contig ID	gi51511721r_77716810
PolyA signal sequence (ATTAAA,-22)	+--------+--------+----*----+---- TCCCCAGATCTTAATTAAACCAGATTTTGCAATTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTCTTATTCTTTCAAGTCTGAGTATACTGGCTTCAATATGATGTGTATA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	r	77816810	77841687	2	99.1	Perfect prediction

Length: 193 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q6ZUX7	5e-80	100.0	Lipoma HMGIC fu...	Homo sapiens
BAC86089	5e-80	100.0	unnamed protein...	Homo sapiens
XP_001108010	2e-79	99.0	similar to lipo...	Macaca mulatta
AAI42331	1.8e-73	92.7	LHFPL2 protein ...	Bos taurus
XP_001517941	4e-65	83.9	similar to HMGI...	Ornithorhynchus...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	66	AIFLAVGIFILCMVALVSVFTMC	88	PRIMARY	23
	2	100	VCGLLQGIAGLFLILGLILYPAG	122	PRIMARY	23
	3	147	GWAFYTAIGGTVLTFICAVFSAQ	169	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	ACTCGGCACCACTTGATATTC
Primer_r	TGCACAGGAACCAAATCAATG
PCR product length	150 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles