KIAA0268

Product ID	ORK05980
Accession No	D87742
Description	melanoma inhibitory activity family, member 3
Clone name	ha07061
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5976 bp) Predicted protein sequence (1193 aa)
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0268 by Kazusa Mouse cDNA Project

Length: 5976 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2394 bp
Genome contig ID	gi89161185f_220769328
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- TATATTCAGGTCTGAATTAAAGTTAAGTTAATCAC
Flanking genome sequence (138645 - 138694)	----+--------+--------+--------+--------+----* ACAGTGTTCAATTTAAGCTTCTTTAATGTTGATGAAAGGTATTTGTAGTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	220869328	220907971	25	99.4	Perfect prediction

Length: 1193 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW93268	99.9	hCG25333, isofo...	Homo sapiens
Q5JRA6	99.9	Melanoma inhibi...	Homo sapiens
XP_001100730	92.6	similar to mela...	Macaca mulatta
XP_514216	90.2	melanoma inhibi...	Pan troglodytes
XP_536152	79.3	similar to mela...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	429	ENAILLIYSFMFYLTKSLVATLP	451	SECONDARY	23
SOSUI2	2	469	FITAFLGIASFAIFLWRTVLVVK	491	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	ATCATCCCTCAGCCAAATCAC
Primer_r	ACACAGAATATACCACATCCC
PCR product length	234 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles