KIAA0260

Product ID	ORK01063
Accession No	D87449
Description	solute carrier family 35 (UDP-GlcA/UDP-GalNAc transporter), member D1
Clone name	ha07062
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5918 bp) Predicted protein sequence (383 aa)
HaloTag ORF Clone	FHC01063
Flexi ORF Clone	FXC01063
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0260 by Kazusa Mouse cDNA Project

Length: 5918 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	4764 bp
Genome contig ID	gi89161185r_67137653
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- TTTTTGTAATGGTGTAAAATAAATACTTTTTCCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAACAAGTATTAAAATTCCTTTGTAACTGCATACAGAGACTAATCCAGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	67237653	67292370	13	100.0	Perfect prediction

Length: 383 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001151573	8.7e-150	99.7	solute carrier ...	Pan troglodytes
Q9NTN3	3.3e-139	100.0	UDP-glucuronic ...	Homo sapiens
Q5RDC9	7.1e-139	99.7	UDP-N-acetylglu...	Pongo abelii
XP_001093420	1.7e-138	99.4	similar to solu...	Macaca mulatta
A2VE55	5.3e-136	97.5	UDP-N-acetylglu...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004853	189	357	PF03151	Protein of unknown function DUF250

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	64	TLTVFLKLLAAGFYGVSSFLIVV	86	SECONDARY	23
	2	99	SSLCVGLGQMVATVAVLWVGKAL	121	PRIMARY	23
	3	137	TFPLPLLYFGNQITGLFST	155	SECONDARY	19
	4	159	NLPMFTVLRRFSILFTMFAEGVL	181	SECONDARY	23
	5	186	FSWGIKMTVFAMIIGAFVAASSD	208	PRIMARY	23
	6	212	DLEGYAFILINDVLTAANGAYVK	234	SECONDARY	23
	7	245	YGLLYYNALFMILPTLAIAYFTG	267	SECONDARY	23
	8	281	TLFLLQFTLSCVMGFILMYATVL	303	PRIMARY	23
	9	311	LTTTIVGCIKNILITYIGMVFGG	333	SECONDARY	23
	10	340	TNFIGLNISIAGSLVYSYITFTE	362	SECONDARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	TTGAAAGCACTTGACCCCATG
Primer_r	CATCTTGAAAGTAAGACCTAG
PCR product length	121 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles