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Order Kazusa clone(s) from : |
| Product ID | ORK05414 |
|---|---|
| Accession No | AB014598 |
| Description | hephaestin, transcript variant 2 |
| Clone name | hg00360s1 |
| Vector information | |
| cDNA sequence | DNA sequence (4228 bp) Predicted protein sequence (1104 aa) |
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HaloTag ORF Clone |
FHC05414
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| Flexi ORF Clone | FXC05414 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0698
by Kazusa Mouse cDNA Project
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| Note | We replaced hg00360, former representative clones for KIAA0698 with hg00360s1. (2002/5/10) |
Length: 4228 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 714 bp |
|---|---|
| Genome contig ID | gi89161218f_65200834 |
| PolyA signal sequence (ATTAAA,-24) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (203121 - 203170) |
----+----*----+----*----+----*----+----*----+----* |
Length: 1104 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001117 | 176 | 313 | PF00394 | Multicopper oxidase |
| IPR011707 | 401 | 428 | PF07732 | Multicopper oxidase | |
| IPR011706 | 896 | 1012 | PF07731 | Multicopper oxidase | |
| ScanRegExp | IPR002355 | 287 | 307 | PS00079 | Multicopper oxidase |
| IPR002355 | 639 | 659 | PS00079 | Multicopper oxidase | |
| IPR002355 | 986 | 1006 | PS00079 | Multicopper oxidase | |
| IPR002355 | 991 | 1002 | PS00080 | Multicopper oxidase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 1054 | LASVLVAISVTLLLVVLALGGVV | 1076 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | CTCAACATTCCTTTCAGTGCC |
|---|---|
| Primer_r | TGAAGGTAGGCTGAGTATTGG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CTCAACATTCCTTTCAGTGCC |
| Primer_r | TGAAGGTAGGCTGAGTATTGG |
| PCR product length | 183 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |