KIAA1176

Product ID	ORK06843
Accession No	AB033002
Description	solute carrier family 12 (potassium/chloride transporter), member 5
Clone name	hg02851a
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3305 bp) Predicted protein sequence (1101 aa)
Source	Human adult brain
Rouge ID	mKIAA1176 by Kazusa Mouse cDNA Project

Length: 3305 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	0 bp
Genome contig ID	gi51511747f_43996993
PolyA signal sequence (None)	+--------+--------+----*----+---- TGGACCGGGTGATGCTGGTCCGCGGCGGCGGCCGC
Flanking genome sequence (122636 - 122685)	----+--------+--------+--------+--------+----* GAGGTCATCACCATCTACTCCTGAGAACCAGGACCTGCCACCCGGGCCCG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	20	f	44091413	44119627	25	99.5	Perfect prediction

Length: 1101 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAH74054	99.9	solute carrier ...	Homo sapiens
AAG43493	99.9	electroneutral ...	Homo sapiens
BAF82447	99.8	unnamed protein...	Homo sapiens
XP_001104798	99.3	similar to solu...	Macaca mulatta
EAW75773	99.5	solute carrier ...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000076	15	21	PR01081	K-Cl co-transporter
	IPR000076	35	43	PR01081	K-Cl co-transporter
	IPR000076	236	248	PR01081	K-Cl co-transporter
	IPR000076	271	288	PR01081	K-Cl co-transporter
	IPR000076	512	528	PR01081	K-Cl co-transporter
	IPR000076	538	546	PR01081	K-Cl co-transporter
HMMPfam	IPR004841	94	668	PF00324	Amino acid permease-associated region
HMMTigr	IPR004842	13	1101	TIGR00930	Na-K-Cl cotransporter superfamily

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	89	MGTFMGVYLPCLQNIFGVILFLR	111	PRIMARY	23
	2	123	ESFCMVFICCSCTMLTAISMSAI	145	PRIMARY	23
	3	166	PEFGGAVGLCFYLGTTFAGAMYI	188	SECONDARY	23
	4	193	EILLAYLFPAMAIFKAEDASGEA	215	SECONDARY	23
	5	224	VYGTCVLTCMATVVFVGVKYVNK	246	PRIMARY	23
	6	250	VFLGCVILSILAIYAGVIKSAFD	272	PRIMARY	23
	7	389	TSYFTLLVGIYFPSVTGIMAGSN	411	SECONDARY	23
	8	428	ILAIATTSAVYISSVVLFGACIE	450	PRIMARY	23
	9	465	NLVVGTLAWPSPWVIVIGSFFST	487	SECONDARY	23
	10	528	WALLLTACICEIGILIASLDEVA	550	PRIMARY	23
	11	553	LSMFFLMCYMFVNLACAVQTLLR	575	PRIMARY	23
	12	595	MSLCLALMFICSWYYALVAMLIA	617	PRIMARY	23

Primer_f	GCAACCCCAAGGAAAGCAGTC
Primer_r	TTCATGCTCCCTACTTCCCTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GCAACCCCAAGGAAAGCAGTC
Primer_r	AAGGAGGACACCATAGGGCTG
PCR product length	124 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles