KIAA1120

Product ID	ORK04416
Accession No	AB032946
Description	calcium channel, voltage-dependent, T type, alpha 1I subunit
Clone name	hg02941
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6405 bp) Predicted protein sequence (1024 aa)
Source	Human adult brain

Length: 6405 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3329 bp
Genome contig ID	gi89161203f_38290042
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- AGTGTTGTTTTGAATAAAAGCCCAGAAGCCTATTT
Flanking genome sequence (125641 - 125690)	----+--------+--------+--------+--------+----* AAGAATTTCTCCGTGTGTCTCTGCTGTTCTTTCCCCAGCGGGGTGGGTGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	22	f	38390041	38415681	18	99.4	Perfect prediction

Length: 1024 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAQ09518	100.0	calcium channel...	Homo sapiens
AAM67414	100.0	voltage-depende...	Homo sapiens
Q9P0X4	100.0	Voltage-depende...	Homo sapiens
EAW60347	99.9	calcium channel...	Homo sapiens
EAW60349	99.9	calcium channel...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032949	4.4e-85	54.8	KIAA1123
AB037777	4.4e-11	31.7	KIAA1356

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR002077	94	109	PR00167	Ca2+ channel
FPrintScan	IPR002077	460	486	PR00167	Ca2+ channel
HMMPfam	IPR005821	7	231	PF00520	Ion transport
HMMPfam	IPR005821	322	530	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	9	NYIFTAIFVGEMTLKVVSLGLYF	31	SECONDARY	23
	2	48	LVFVSIIDIVVSLASAGGAKILG	70	PRIMARY	23
	3	107	IGNIVLICCAFFIIFGILGVQLF	129	PRIMARY	23
	4	168	GQALMSLFVLASKDGWVNIMYNG	190	SECONDARY	23
	5	210	LYFISFLLIVSFFVLNMFVGVVV	232	PRIMARY	23
	6	288	HYLDIFITFIICLNVVTMSLEH	309	PRIMARY	22
	7	323	CNYMFTTVFVLEAVLKLVAFGL	344	SECONDARY	22
	8	351	RWNQLDLAIVLLSVMGITLEEIE	373	PRIMARY	23
	9	377	ALPINPTIIRIMRVLRIARVLKL	399	SECONDARY	23
	10	423	LGLLFMLLFFIYAALGVELFGKL	445	PRIMARY	23
	11	506	VSPLYFVSFVLTAQFVLINVVVA	528	PRIMARY	23

Primer_f	TTAGCCCAGATACACATACTC
Primer_r	AGCGCCATCAGCAACTAGGAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TTAGCCCAGATACACATACTC
Primer_r	AGCGCCATCAGCAACTAGGAG
PCR product length	209 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles