KIAA0626

Product ID	ORK00579
Accession No	AB014526
Description	microfibrillar-associated protein 3-like, transcript variant 1
Clone name	hh00753
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6184 bp) Predicted protein sequence (433 aa)
HaloTag ORF Clone	FHC00579
Flexi ORF Clone	FXC00579
Source	Human adult brain
Rouge ID	mKIAA0626 by Kazusa Mouse cDNA Project

Length: 6184 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	4776 bp
Genome contig ID	gi89161207r_171044328
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- GATTGATGCTTTAATAAAGGGTTTGCACAGCTGTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TGGAATGTGTGTTGTGTCCCACCTGAAGGGTGTGTAACTGTGGCTTGACT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	4	r	171144328	171184004	3	100.0	Perfect prediction

Length: 433 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O75121	4e-156	100.0	Microfibrillar-...	Homo sapiens
XP_001154389	8.2e-156	99.8	microfibrillar-...	Pan troglodytes
AAS55434	9.4e-156	99.8	HSD-39 [Homo sa...	Homo sapiens
XP_001084068	1.5e-155	99.8	microfibrillar-...	Macaca mulatta
XP_534551	1.7e-147	90.3	similar to Micr...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013098	71	166	PF07679	Immunoglobulin I-set
HMMSmart	IPR003599	77	167	SM00409	Immunoglobulin subtype
HMMSmart	IPR003598	83	156	SM00408	Immunoglobulin subtype 2
ProfileScan	IPR007110	71	165	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	29	KSHLTVCFLPSVPFLILVSTLAT	51	PRIMARY	23
	2	59	TLNGTNVVLGSVPVIIARTDHII	81	SECONDARY	23
	3	177	YMVVCLVAFTIVMVLNITRLCMM	199	PRIMARY	23

Primer_f	TAGAAAGGCAAACCAGATACC
Primer_r	ACTCAATGCCAAAAGGGTACG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TAGAAAGGCAAACCAGATACC
Primer_r	ACTCAATGCCAAAAGGGTACG
PCR product length	229 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles