KIAA1191

Gene/Protein Characteristic Table for KIAA1191

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Product ID	ORK05641
Accession No	AB033017
Description	KIAA1191
Clone name	hh01795a
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (1837 bp) Predicted protein sequence (100 aa)
Source	Human adult brain
Rouge ID	mKIAA1191 by Kazusa Mouse cDNA Project

Features of the cloned cDNA sequence

Description

Length: 1837 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1533 bp
Genome contig ID	gi51511721r_175605674
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- CTCGTTTTGACAATAAAAATCCTTACTACTTTCTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAATGTGGCTATTTTTTTGTCCTTTTAACAATAGTTATTCCACTCTACCC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	r	175705674	175707624	2	99.1	Perfect prediction

Features of the protein sequence

Description

Length: 100 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB14957	4.6e-38	100.0	unnamed protein...	Homo sapiens
CAB99231	2.4e-37	99.0	hypothetical pr...	Homo sapiens
CAH92202	5.6e-37	98.0	hypothetical pr...	Pongo abelii
XP_001083749	5.9e-36	95.0	hypothetical pr...	Macaca mulatta
XP_866160	1.8e-33	88.0	hypothetical pr...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Expression profile

Description

RT-PCR-ELISA

Experimental conditions

Primer_f	TGCTCCAGCCTTATCAGTTAG
Primer_r	ATGAAGAACTAGCTGCAAGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information

Description

Chromosome No. 5

Experimental conditions

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-

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