|
Order Kazusa clone(s) from : |
| Product ID | ORK02006 |
|---|---|
| Accession No | AB023161 |
| Description | dynein, axonemal, heavy chain 7 |
| Clone name | hj04408s1 |
| Vector information | |
| cDNA sequence | DNA sequence (12418 bp) Predicted protein sequence (4031 aa) |
|
HaloTag ORF Clone |
FHC02006
|
| Flexi ORF Clone | FXC02006 |
| Source | Human adult brain |
| Note | We replaced hj04408, former representative clones for KIAA0944 with hj04408s1. (2002/12/27) |
Length: 12418 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 4031 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR013602 | 761 | 1174 | PF08393 | Dynein heavy chain |
| IPR011704 | 1611 | 1762 | PF07728 | ATPase associated with various cellular activities | |
| IPR011704 | 1978 | 2125 | PF07728 | ATPase associated with various cellular activities | |
| IPR004273 | 3319 | 4028 | PF03028 | Dynein heavy chain | |
| HMMSmart | IPR003593 | 1327 | 1466 | SM00382 | AAA+ ATPase |
| IPR003593 | 1975 | 2123 | SM00382 | AAA+ ATPase | |
| ProfileScan | IPR002048 | 2247 | 2282 | PS50222 | Calcium-binding EF-hand |
| ScanRegExp | IPR002048 | 2260 | 2272 | PS00018 | Calcium-binding EF-hand |
| IPR002198 | 3447 | 3475 | PS00061 | Short-chain dehydrogenase/reductase SDR |
RT-PCR-ELISA
|
Experimental conditions| Primer_f | TCTGACCAACCCAAGGAACAC |
|---|---|
| Primer_r | TCTACTCAGCCAGCCAACAAG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 2
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TCTGACCAACCCAAGGAACAC |
| Primer_r | TCTACTCAGCCAGCCAACAAG |
| PCR product length | 117 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |