KIAA0958

Gene/Protein Characteristic Table for KIAA0958

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Product ID	ORK00697
Accession No	AB023175
Description	protein O-fucosyltransferase 2, transcript variant 1
Clone name	hj05707
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4804 bp) Predicted protein sequence (428 aa)
HaloTag ORF Clone	FHC00697
Flexi ORF Clone	FXC00697
Source	Human adult brain

Length: 4804 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3515 bp
Genome contig ID	gi51511750r_45408280
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- ACCTTCCTTTTTAAGAAAAATAAACTGCTTTATGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAGTTGGTAGCTGTGGAGTGTAGACTCATTAGACTCATTTCCTGCCACAC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	21	r	45508280	45532228	8	99.2	Perfect prediction

Features of the protein sequence

Description

Length: 428 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
CAC24557	1.7e-190	100.0	C21orf80 protei...	Homo sapiens
T14795	6.2e-170	100.0	5c02f12-t7 memb...	Zea mays
Q9Y2G5	4.4e-169	98.2	GDP-fucose prot...	Homo sapiens
EAX09353	7.8e-169	100.0	protein O-fucos...	Homo sapiens
EAX09351	8e-169	100.0	protein O-fucos...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	5	MATLSFVFLLLGAVSWPP	22	PRIMARY	18

Expression profile

Description

RT-PCR-ELISA

Experimental conditions

Primer_f	CAGGGTCCTATGGTGTTACTC
Primer_r	CAACTGATGAAAAAGGCACTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information

Description

Chromosome No. 21

Experimental conditions

Panel name	GeneBridge 4
Primer_f	CAGGGTCCTATGGTGTTACTC
Primer_r	CAACTGATGAAAAAGGCACTG
PCR product length	98 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles

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