KIAA1919

Product ID	ORK05728
Accession No	AB067506
Description	KIAA1919
Clone name	hj08057
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5261 bp) Predicted protein sequence (403 aa)
Source	Human adult brain

Length: 5261 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	4047 bp
Genome contig ID	gi89161210f_111593802
PolyA signal sequence (None)	+--------+--------+----*----+---- ACTCCAGCCTGGGCAACAGAGGGAGACTCCATCTC
Flanking genome sequence (105263 - 105312)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAAAAAAAGGAAATACAGTTGAATATGAGGGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	6	f	111693802	111699063	1	99.0	Perfect prediction

Length: 403 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAG52663	1.5e-157	99.8	unnamed protein...	Homo sapiens
EDL87827	8e-110	74.2	rCG19957 [Rattu...	Rattus norvegicus
XP_574786	9.1e-110	74.2	similar to Na+ ...	Rattus norvegicus
EDL04934	3.6e-104	76.9	mCG1413 [Mus mu...	Mus musculus
EDL87830	2.7e-98	79.9	similar to Na+ ...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	78	263	PF07690	Major facilitator superfamily MFS_1

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	9	ALHFSFALGAFLAPLLAKLALGP	31	SECONDARY	23
	2	69	WAYAVIGTYMFLVSVIFFCLFLK	91	PRIMARY	23
	3	110	KYHNALLCLLFLFFFFYVGAEVT	132	PRIMARY	23
	4	153	AGLNSIFWGTFAACRGLAIFFAT	175	SECONDARY	23
	5	180	GTMIVLSNIGSLTSSLFLVLFDK	202	SECONDARY	23
	6	209	IATSVYGASMATTFPSGVSWIEQ	231	SECONDARY	23
	7	239	SAAFFVIGASLGEMAIPAVIGIL	261	SECONDARY	23
	8	271	VLYTSLGASIATGILFPVLYKLA	293	PRIMARY	23

Primer_f	GACAGAAAGAGTGAGGACCAG
Primer_r	GATTATAGACTTCAGCTGTGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-