KIAA0653

Gene/Protein Characteristic Table for KIAA0653

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Product ID	ORK05481
Accession No	AB014553
Description	inducible T-cell co-stimulator ligand
Clone name	hk01718
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4358 bp) Predicted protein sequence (558 aa)
Source	Human adult brain
Rouge ID	mKIAA0653 by Kazusa Mouse cDNA Project

Length: 4358 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Features of the protein sequence

Description

Length: 558 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAX09447	4.1e-161	91.3	inducible T-cel...	Homo sapiens
O75144	1.2e-100	100.0	ICOS ligand; B7...	Homo sapiens
AAF34739	3.2e-100	100.0	B7-like protein...	Homo sapiens
XP_531578	1.1e-98	98.3	similar to B7-l...	Pan troglodytes
BAH11654	3.6e-70	100.0	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013106	57	173	PF07686	Immunoglobulin V-set
HMMSmart	IPR003599	64	177	SM00409	Immunoglobulin subtype
ProfileScan	IPR007110	36	171	PS50835	Immunoglobulin-like
ProfileScan	IPR007110	183	269	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	297	ATWSILAVLCLLVVVAVAIGWVC	319	PRIMARY	23

Expression profile

Description

RT-PCR

RT-PCR-ELISA

Experimental conditions

Primer_f	AGCTTCTGTCCCTCATGCCTG
Primer_r	GGGTAGGTCTCACAAATGCCG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information

Description

Chromosome No. 21

Experimental conditions

Panel name	GeneBridge 4
Primer_f	AGCTTCTGTCCCTCATGCCTG
Primer_r	GGGTAGGTCTCACAAATGCCG
PCR product length	172 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles

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