KIAA0805

Product ID	ORK05622
Accession No	AB018348
Description	pecanex homolog (Drosophila)
Clone name	hk02940s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pYO326 vector. The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. pYO326 shuttle vector, pYO326 (Amp-r) was kindly provided by Y. Ohya (University of Tokyo).
cDNA sequence	DNA sequence (4204 bp) Predicted protein sequence (1327 aa)
Source	Human adult brain
Rouge ID	mKIAA0805 by Kazusa Mouse cDNA Project
Note	We replaced hk02940, former representative clones for KIAA0805 with hk02940s1. (2002/5/10)

Length: 4204 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	218 bp
Genome contig ID	gi51511730f_70449501
PolyA signal sequence (AATAAA,-27)	+--------+--------+----*----+---- CATCCCCAAATAAAGCTGAAATATTTTTTTAAGTT
Flanking genome sequence (197124 - 197173)	----+--------+--------+--------+--------+----* AGCTGCCGAGAAAACATTTTGCATGAAGGATAAAGTTCTGTTAAAATACA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	14	f	70549501	70646623	25	99.9	Perfect prediction

Length: 1327 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW81053	99.9	pecanex homolog...	Homo sapiens
XP_001787908	86.3	similar to rCG2...	Bos taurus
XP_866477	61.9	similar to peca...	Canis lupus fam...
AAK82958	94.9	pecanex-like pr...	Homo sapiens
AAI40785	94.8	PCNX protein [H...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007895	3.2e-93	53.6	KIAA0435

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR007735	765	995	PF05041	Pecanex

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	34	FWILPQLWIGINFDRLTLLALFD	56	SECONDARY	23
	2	63	ENVLAVILAILVAFLGSILLIQG	85	PRIMARY	23
	3	125	IAYSRPVYFCICCGLIWLLDYGS	147	SECONDARY	23
	4	172	RDLVIVFTLCFPIVFFIGLLPQV	194	PRIMARY	23
	5	219	LAALYSFICSIVAVALLYGLCYG	241	PRIMARY	23
	6	249	GQHIPVLFSIFCGLLVAVSYHLS	271	PRIMARY	23
	7	322	SDLVVCIVIGVLYFAIHVSTVFT	344	PRIMARY	23
	8	350	LKYVLYTLVGFVGFVTHYVLPQV	372	PRIMARY	23
	9	411	WLLFVEKNIIYPLIVLNELSSSA	433	SECONDARY	23
	10	495	LFFMSILFNKLWELLYKLQFVYT	517	SECONDARY	23
	11	536	PFAVPHSAMLFIQAAVSAFFSTP	558	SECONDARY	23
	12	560	NPFLGSAIFITSYVRPVKFWER	581	SECONDARY	22

Primer_f	GGGTACTAGAACTTGGGGCTG
Primer_r	GCTTTATTTGGGGATGGAGAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GGGTACTAGAACTTGGGGCTG
Primer_r	GCTTTATTTGGGGATGGAGAC
PCR product length	228 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles