KIAA0761

Product ID	ORK00125
Accession No	AB018304
Description	chloride channel CLIC-like 1, transcript variant 1
Clone name	hk04655s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4696 bp) Predicted protein sequence (551 aa)
HaloTag ORF Clone	FHC00125
Flexi ORF Clone	FXC00125
Source	Human adult brain
Note	We replaced hk04655, former representative clones for KIAA0761 with hk04655s1. (2002/12/27)

Length: 4696 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3039 bp
Genome contig ID	gi89161185r_109173653
PolyA signal sequence (AAGAAA,-21)	+--------+--------+----*----+---- AGCCTGATTTCTGAAAGAAATAATCTTTAAACCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAACTGCATAAAATTTTTCCTTTAAAATGGCTTATATAGGTATAGCTGCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	109273653	109294583	11	99.3	Perfect prediction

Length: 551 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_513612	0	99.3	Mid-1-related c...	Pan troglodytes
XP_001087523	3.4e-212	96.4	similar to Mid-...	Macaca mulatta
Q1LZF8	3.4e-177	82.0	Chloride channe...	Bos taurus
XP_001927252	3.5e-173	79.5	chloride channe...	Sus scrofa
AAH81736	6.2e-156	73.2	Clcc1 protein [...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR009231	3	551	PF05934	Mid-1-related chloride channel

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	MLCSLLLCECLLLVAGYAHDDDW	23	PRIMARY	23
	2	181	DPYNVLMVLLCLLCIVVLVATEL	203	PRIMARY	23
	3	216	VLIISFLFSLGWNWMYLYKLAFA	238	SECONDARY	23
	4	329	EIPALLHLPVLIIMALAILSFCY	351	PRIMARY	23

Primer_f	CTGTTCATCCATTCTCTTTCC
Primer_r	GACTGAGAGATTTAACATAGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTGTTCATCCATTCTCTTTCC
Primer_r	GACTGAGAGATTTAACATAGC
PCR product length	197 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles