KIAA1094

Product ID	ORK00739
Accession No	AB029017
Description	dolichol kinase
Clone name	hk06464a
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2089 bp) Predicted protein sequence (544 aa)
HaloTag ORF Clone	FHC00739
Flexi ORF Clone	FXC00739
Source	Human adult brain
Rouge ID	mKIAA1094 by Kazusa Mouse cDNA Project

Length: 2089 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	156 bp
Genome contig ID	gi89161216r_130647631
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TGATTCAGATTTAAAATAAAAAGCAAAGCTCTCCT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTCTAGTTTTGCTGATATATTTTTTTTTTTATTTTTTTTTGAGACAGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	130747631	130749719	1	99.0	Perfect prediction

Length: 544 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9UPQ8	1.9e-206	100.0	Dolichol kinase...	Homo sapiens
ABY56110	1.1e-205	99.6	dolichol kinase...	Papio anubis
BAE91315	3.3e-205	99.4	unnamed protein...	Macaca fascicularis
ACA57898	1.6e-204	98.9	transmembrane p...	Callicebus moloch
ABY84169	1.3e-202	98.1	transmembrane p...	Callithrix jacchus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000374	341	543	PF01148	Phosphatidate cytidylyltransferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	LSGSVLAEAAVVFAVVLSIHATV	44	PRIMARY	23
	2	114	ERFGIVVAATGMAVALFSSVLAL	136	PRIMARY	23
	3	142	VPTNTCVILGLAGGVIIYIMKHS	164	PRIMARY	23
	4	170	VIEVLEVLLIFVYLNMILLYLLP	192	PRIMARY	23
	5	194	CFTPGEALLVLGGISFVLNQLI	215	SECONDARY	22
	6	229	VDFFLLVVVVGMVLMGIFFSTLF	251	PRIMARY	23
	7	264	FFHLMTCVLSLGVVLPWLHRLI	285	SECONDARY	22
	8	305	YLLAYWSLLATLACLVVLYQNA	326	PRIMARY	22
	9	352	ATYIPGIIFDRPLLYVAATVCLA	374	PRIMARY	23
	10	408	GPLILTHIYLLLGMSLPIWLIPR	430	SECONDARY	23
	11	442	RALVPYAGVLAVGVGDTVASIFG	464	SECONDARY	23
	12	481	GTMTSIFAQIISVALILIFDSGV	503	SECONDARY	23
	13	516	ISTVSLLEAYTTQIDNLLLPLYL	538	PRIMARY	23

Primer_f	CTCTACCTCCTGATATTGCTG
Primer_r	TCACACCTTGGCTCTTCATGC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTCTACCTCCTGATATTGCTG
Primer_r	TCACACCTTGGCTCTTCATGC
PCR product length	129 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles