KIAA0880

Product ID	ORK01613
Accession No	AB020687
Description	solute carrier organic anion transporter family, member 2B1, transcript variant 1
Clone name	hk07457
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4068 bp) Predicted protein sequence (727 aa)
HaloTag ORF Clone	FHC01613
Flexi ORF Clone	FXC01613
Source	Human adult brain
Rouge ID	mKIAA0880 by Kazusa Mouse cDNA Project

Length: 4068 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1672 bp
Genome contig ID	gi51511727f_74439809
PolyA signal sequence (ATTAAA,-19)	+--------+--------+----*----+---- ATAGATACAGCTTATTATTAAATTTGTTCTTGCAT
Flanking genome sequence (155138 - 155187)	----+--------+--------+--------+--------+----* AATGTCTCTTCTATTACAAAAATTCTTTCTTCATAAACTGCATTAGAGGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	f	74539809	74594945	14	99.8	Perfect prediction

Length: 727 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O94956	100.0	Solute carrier ...	Homo sapiens
BAF82923	99.9	unnamed protein...	Homo sapiens
XP_001174975	99.2	solute carrier ...	Pan troglodytes
XP_522110	98.6	solute carrier ...	Pan troglodytes
BAG57720	100.0	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004156	66	666	PF03137	Organic anion transporter polypeptide OATP
HMMTigr	IPR004156	35	693	TIGR00805	Organic anion transporter polypeptide OATP

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	62	VFHNIKLFVLCHSLLQLAQLMIS	84	SECONDARY	23
	2	135	MIGYGAILVALAGLLMTLPHFIS	157	PRIMARY	23
	3	207	VGIMFVAQTLLGVGGVPIQPFGI	229	SECONDARY	23
	4	244	YLGILFAVTMMGPGLAFGLGSLM	266	SECONDARY	23
	5	293	AWWLGFLIAAGAVALAAIPYFFF	315	PRIMARY	23
	6	393	LVVLSQVCLSSMAAGMATFLPKF	415	SECONDARY	23
	7	427	ANLLIGCLSFPSVIVGIVVGGVL	449	PRIMARY	23
	8	459	GCGALCLLGMLLCLFFSLPLFFI	481	PRIMARY	23

Primer_f	GAGCATGTATCTATCAACGCC
Primer_r	CTGCAAAACCTGGGAAACAAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTGCAAAACCTGGGAAACAAG
Primer_r	GAGCATGTATCTATCAACGCC
PCR product length	105 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles