KIAA0995

Product ID	ORK06296
Accession No	AB023212
Description	pecanex homolog (Drosophila)
Clone name	hk07740
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4410 bp) Predicted protein sequence (1014 aa)
Source	Human adult brain

Length: 4410 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1364 bp
Genome contig ID	gi51511730f_70343875
PolyA signal sequence (None)	+--------+--------+----*----+---- ATTGCACCACTGTACTTCAGACAAGACTCTGTCTC
Flanking genome sequence (205415 - 205464)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAGTGTATGAGAGAAGAAAATTAAAGGGCTTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	14	f	70443875	70549288	10	99.9	Perfect prediction

Length: 1014 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW81052	99.9	pecanex homolog...	Homo sapiens
EAW81055	99.9	pecanex homolog...	Homo sapiens
EAW81053	99.9	pecanex homolog...	Homo sapiens
AAK82958	99.9	pecanex-like pr...	Homo sapiens
Q96RV3	99.9	Pecanex-like pr...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB011125	0.0002	20.2	KIAA0553

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	80	LLLSGLLLLVCCLLLLLQQ	98	PRIMARY	19
	2	130	GAGAGTAAAAAATAAAPGGDGVA	152	SECONDARY	23
	3	181	RAAPLPVALLLGLPFTLYMALPS	203	PRIMARY	23
	4	207	IVAVYCPVIAAVFIVLKMVNYRL	229	PRIMARY	23

Primer_f	GTGGGAAGAAATGAGAGTTGC
Primer_r	TCGTTAGTGGCTTCTCTTCTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GTGGGAAGAAATGAGAGTTGC
Primer_r	TCGTTAGTGGCTTCTCTTCTC
PCR product length	124 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles