|
Order Kazusa clone(s) from : |
| Product ID | ORK00209 |
|---|---|
| Accession No | AB033099 |
| Description | ATPase family, AAA domain containing 3B |
| Clone name | hk09394 |
| Vector information | |
| cDNA sequence | DNA sequence (4334 bp) Predicted protein sequence (606 aa) |
|
HaloTag ORF Clone |
FHC00209
|
| Flexi ORF Clone | FXC00209 |
| Source | Human adult brain |
| Rouge ID |
mKIAA1273
by Kazusa Mouse cDNA Project
|
Length: 4334 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 1 | f | 1403269 | 1423094 | 14 | 99.5 | Terminal No-hit |
Length: 606 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR003959 | 305 | 501 | PF00004 | AAA ATPase |
| HMMSmart | IPR003593 | 302 | 435 | SM00382 | AAA+ ATPase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 3 | VQMQLEALNLLHTLVWARSLCRA | 25 | SECONDARY | 23 | 2 | 204 | KVTATVAGLTLLAVGVYSAKNAT | 226 | PRIMARY | 23 |
|---|
RT-PCR-ELISA
|
Experimental conditions| Primer_f | GAATCACAGCAACGAGACAGC |
|---|---|
| Primer_r | ACTTCAACCAACAGCCTACAC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 1
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GAATCACAGCAACGAGACAGC |
| Primer_r | ACTTCAACCAACAGCCTACAC |
| PCR product length | 114 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |