|
Order Kazusa clone(s) from : |
| Product ID | ORK00680 |
|---|---|
| Accession No | AB020715 |
| Description | prenylcysteine oxidase 1 |
| Clone name | hk10449 |
| Vector information | |
| cDNA sequence | DNA sequence (4226 bp) Predicted protein sequence (506 aa) |
|
HaloTag ORF Clone |
FHC00680
|
| Flexi ORF Clone | FXC00680 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0908
by Kazusa Mouse cDNA Project
|
Length: 4226 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 2 | f | 70338797 | 70360734 | 6 | 99.2 | Perfect prediction |
Length: 506 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR013027 | 37 | 74 | PF07992 | FAD-dependent pyridine nucleotide-disulphide oxidoreductase |
| IPR010795 | 129 | 506 | PF07156 | Prenylcysteine lyase |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 4 | RVVAELVSSLLGLWLLLCSCGCP | 26 | PRIMARY | 23 | 2 | 36 | DKIAIIGAGIGGTSAAYYLR | 55 | SECONDARY | 20 | 3 | 130 | EESNWFIINVIKLVWRYGFQFLR | 152 | SECONDARY | 23 |
|---|
RT-PCR-ELISA
|
Experimental conditions| Primer_f | GGGAAAGATGTGAAGATAGAC |
|---|---|
| Primer_r | TAAAGGATGGATGACAGAACC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 2
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GGGAAAGATGTGAAGATAGAC |
| Primer_r | TAAAGGATGGATGACAGAACC |
| PCR product length | 114 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |