FLJ00130

Product ID	ORK04461
Accession No	AK074059
Clone name	sh01916
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5209 bp) Predicted protein sequence (294 aa)
Source	Human spleen
Rouge ID	mFLJ00130 by Kazusa Mouse cDNA Project

Length: 5209 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 294 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84885	2.7e-97	100.0	FLJ00130 protei...	Homo sapiens
EAW89749	4.6e-97	99.7	hCG1773689, iso...	Homo sapiens
AAH40264	1.6e-96	99.3	Coiled-coil dom...	Homo sapiens
NP_932348	2.8e-95	99.7	coiled-coil dom...	Homo sapiens
Q2TAC2	2.8e-95	98.3	Coiled-coil dom...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Primer_f
Primer_r
PCR conditions	test