FLJ00136

Product ID	ORK01035
Accession No	AK074065
Clone name	sh02543
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5291 bp) Predicted protein sequence (811 aa)
HaloTag ORF Clone	FHC01035
Flexi ORF Clone	FXC01035
Source	Human spleen
Rouge ID	mFLJ00136 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 811 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84891	100.0	FLJ00136 protei...	Homo sapiens
Q7Z403	100.0	Transmembrane c...	Homo sapiens
AAH23597	99.9	Transmembrane c...	Homo sapiens
AAL25836	99.9	Evin1 [Homo sap...	Homo sapiens
AAP69874	99.8	transmembrane c...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK090478	9.1e-17	29.6	FLJ00400

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	215	CVLALHSLGLALLSALQALMPWR	237	PRIMARY	23
	2	253	SYFLFLKTLLAFNALLLLLLVAF	275	PRIMARY	23
	3	348	AYLSTVGVSFFITCITLVYSMAH	370	PRIMARY	23
	4	442	LVWLLCLGTALGCAVAVHVFSEF	464	PRIMARY	23
	5	479	LVLPLVVGLLNLGAPYLCRVLAA	501	PRIMARY	23
	6	515	AICRNLILKLAILGTLCYHWLG	536	SECONDARY	22
	7	558	FLVMDFVLMLLDTLFGELVWRII	580	PRIMARY	23
	8	610	WLGVLFSPLLPAVQIIKLLLVFY	632	PRIMARY	23
	9	655	STVFLTLLCFPAFLGAAVFLCYA	677	PRIMARY	23
	10	725	MENTFFVFLVSALLLAVIYLNIQ	747	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test