FLJ00142

Product ID	ORK06176
Accession No	AK074071
Clone name	sh04107
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5884 bp) Predicted protein sequence (674 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 674 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84897	100.0	FLJ00142 protei...	Homo sapiens
BAB84884	100.0	FLJ00129 protei...	Homo sapiens
EAW77831	100.0	hCG2003451, iso...	Homo sapiens
AAK57194	99.8	NADPH oxidase 5...	Homo sapiens
Q96PH1	99.8	NADPH oxidase 5.	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK074058	0	100.0	FLJ00129

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR002048	46	98	PD000012	Calcium-binding EF-hand
HMMPfam	IPR002048	78	106	PF00036	Calcium-binding EF-hand
HMMPfam	IPR002916	172	673	PF01794	Ferric reductase-like transmembrane component
ProfileScan	IPR002048	46	103	PS50222	Calcium-binding EF-hand
ScanRegExp	IPR002048	87	99	PS00018	Calcium-binding EF-hand

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	148	LFCLATYAGLHVLLFGLAASAH	169	SECONDARY	22
	2	183	GQCLNFDCSFIAVLMLRRCLTWL	205	SECONDARY	23
	3	277	SPTGVALLLLLLFMFICSSSCI	298	PRIMARY	22
	4	315	LLVWLLLIFHGPNFWKWLLVPGI	337	PRIMARY	23
	5	343	KAIGLAVSRMAAVCIMEVNLLPS	365	SECONDARY	23

Primer_f
Primer_r
PCR conditions	test