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Gene/Protein Characteristic Table for FLJ00322

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Product ID	ORK05161
Accession No	AK160377
Clone name	sh08187
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5343 bp) Predicted protein sequence (659 aa)
Source	Human spleen

Length: 5343 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 659 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000203	287	336	PF01825	GPS domain
	IPR001024	394	509	PF01477	Lipoxygenase
HMMSmart	IPR000203	287	336	SM00303	GPS domain
	IPR001024	394	508	SM00308	Lipoxygenase
ProfileScan	IPR000203	288	337	PS50221	GPS domain
	IPR001024	408	496	PS50095	Lipoxygenase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	349	VNYIVMLTCAVCLVTYMVMAAIL	371	PRIMARY	23
	2	553	HIQRATCCVLLICLFLGANAVWY	575	PRIMARY	23
	3	598	TVAVGLVSSVVVYPVYLAILFLF	620	PRIMARY	23

Experimental conditions

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