FLJ00175

Product ID	ORK04849
Accession No	AK074104
Clone name	sj00758
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4645 bp) Predicted protein sequence (1516 aa)
Source	Human spleen
Rouge ID	mFLJ00175 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 1516 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84930	100.0	FLJ00175 protei...	Homo sapiens
EAW99765	99.5	dysferlin, limb...	Homo sapiens
ACB12753	99.6	dysferlin varia...	Homo sapiens
ACB12759	99.6	dysferlin varia...	Homo sapiens
ACB12755	99.6	dysferlin varia...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033033	0	57.2	KIAA1207

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR000008	1143	1155	PR00360	C2 domain
	IPR000008	1169	1182	PR00360	C2 domain
	IPR000008	1191	1199	PR00360	C2 domain
HMMPfam	IPR000008	702	792	PF00168	C2 domain
	IPR000008	882	969	PF00168	C2 domain
	IPR000008	1128	1211	PF00168	C2 domain
HMMSmart	IPR000008	701	809	SM00239	C2 domain
	IPR000008	886	985	SM00239	C2 domain
	IPR000008	1127	1226	SM00239	C2 domain
	IPR000008	1360	1489	SM00239	C2 domain
ProfileScan	NULL	586	645	PS50323	NULL
	IPR000008	687	792	PS50004	C2 domain
	IPR000008	1113	1211	PS50004	C2 domain

Primer_f
Primer_r
PCR conditions	test