FLJ00400

Product ID	ORK01047
Accession No	AK090478
Clone name	sj07486
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4379 bp) Predicted protein sequence (782 aa)
HaloTag ORF Clone	FHC01047
Flexi ORF Clone	FXC01047
Source	Human spleen
Rouge ID	mFLJ00400 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 782 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC03459	0	100.0	FLJ00400 protei...	Homo sapiens
BAG10285	0	100.0	transmembrane c...	synthetic construct
Q8IU68	0	99.7	Transmembrane c...	Homo sapiens
AAI41866	0	99.6	Transmembrane c...	Homo sapiens
XP_591560	2e-212	81.5	similar to FLJ0...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK074065	7.3e-09	29.6	FLJ00136

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	170	RFLLLLNLLSLLLTASFVLLPLV	192	PRIMARY	23
	2	254	VYSIRLAYLLSPLACLLLCFCGT	276	PRIMARY	23
	3	355	YLRVNVLIGLLVVGAISAIFWAT	377	PRIMARY	23
	4	392	LQYLPPGVIALVNFLGPLLFTFL	414	SECONDARY	23
	5	431	IWCVVLKLASLGMFSVSLGQTIL	453	SECONDARY	23
	6	483	LYKLSIFNFLLTVAFAFLVTLP	504	PRIMARY	22
	7	544	GLFYCPLLPLLNSVFLFLTFYI	565	PRIMARY	22
	8	586	STFFFQLVLLLGLLLAAVPLGYV	608	PRIMARY	23
	9	624	YSAPWQVVPELVALGLPPIGQRA	646	SECONDARY	23
	10	654	AFSFPLLIMLSLVLTVCVSQTQA	676	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test