HUGE

Gene/Protein Characteristic Table for KIAA0115

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00413
Accession No. :	D29643
Description :	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor.
HUGO Gene Name :	dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST)
Clone Name :	ha00643 [Vector Info]
Flexi ORF Clone :	pF1KSDA0115
Source :	Myeloblast cell line (KG-1)

Features of the cloned DNA sequence	Description

Length: 1668 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 191 bp

Genome contig ID gi89161185r_20751268
PolyA signal sequence
(TATAAA,-24)
+----*----+----*----+----*----+----
GATAATTTTTATATAAAAGAAGTTTTTCCACTTTG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AATTGCTAAAAGTGGCATTTTTCCTATGTGCAGTCACTCCTCTCATTTCT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 20851268 20860587 11 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 456 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAA23670	5.8e-197	99.8	oligosaccharylt...	Homo sapiens
AAH02594	9.2e-197	99.8	Dolichyl-diphos...	Homo sapiens
AAP36629	9.2e-197	99.8	dolichyl-diphos...	synthetic construct
XP_001161661	3.8e-196	99.6	dolichyl-diphos...	Pan troglodytes
CAH73476	5.2e-196	99.6	dolichyl-diphos...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR005013 26 455 PF03345 Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005013	26	455	PF03345	Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48kDa subunit

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 22 TAARAWALFWLLLPLLGAVCASG 44 PRIMARY 23

2 431 SAFPMMLGLFIFSIVFLHM 449 PRIMARY 19

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	TAARAWALFWLLLPLLGAVCASG	44	PRIMARY	23
2	431	SAFPMMLGLFIFSIVFLHM	449	PRIMARY	19

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: Stanford G3
Primer_f	: GGGGGTTATTAGGATTGGTGG
Primer_r	: ACCCCTTGGCTCTCAGTGTTG
PCR product length	: 103 bp
PCR conditions	: 95 °C15 sec66 °C120 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	191 bp
Genome contig ID	gi89161185r_20751268
PolyA signal sequence (TATAAA,-24)	+--------+--------+----*----+---- GATAATTTTTATATAAAAGAAGTTTTTCCACTTTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AATTGCTAAAAGTGGCATTTTTCCTATGTGCAGTCACTCCTCTCATTTCT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	20851268	20860587	11	99.6	Perfect prediction

Gene/Protein Characteristic Table for KIAA0115

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information