HUGE

Gene/Protein Characteristic Table for KIAA0169

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	D79991
Description :	nucleoporin 188kDa.
HUGO Gene Name :
Clone Name :	ha02315 [Vector Info]
Source :	Myeloblast cell line (KG-1)

Features of the cloned DNA sequence	Description

Length: 5654 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 417 bp

Genome contig ID gi89161216f_130651287
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
CTATTTATACAGATATTAAAATCTTGTTTATAGAC
Flanking genome sequence
(157910 - 157959)
----+----*----+----*----+----*----+----*----+----*
AGCTGTGTGATGTTTAACTTCAAAGCCCAGGGATGACAACGTGGCTCTCA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 f 130749831 130809195 44 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 1745 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q5SRE5	100.0	Nucleoporin NUP...	Homo sapiens
XP_520414	99.7	nucleoporin 188...	Pan troglodytes
ABY56111	99.1	nucleoporin 188...	Papio anubis
ACA57899	97.3	nucleoporin (pr...	Callicebus moloch
XP_001928863	96.2	similar to nucl...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 1026 PSILETCALIMKIICLEIYYVVK 1048 PRIMARY 23

2 1093 LLEYQMLVSAWRMLLIIATTHAD 1115 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1026	PSILETCALIMKIICLEIYYVVK	1048	PRIMARY	23
2	1093	LLEYQMLVSAWRMLLIIATTHAD	1115	SECONDARY	23

Expression profile	Description

: Northern blot Description

Northern blot	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: AGAGGTCAACAGCAGGCATGG
Primer_r	: TTGAAACCCGTGCATAGAGCC
PCR product length	: 162 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	417 bp
Genome contig ID	gi89161216f_130651287
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- CTATTTATACAGATATTAAAATCTTGTTTATAGAC
Flanking genome sequence (157910 - 157959)	----+--------+--------+--------+--------+----* AGCTGTGTGATGTTTAACTTCAAAGCCCAGGGATGACAACGTGGCTCTCA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	f	130749831	130809195	44	100.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA0169

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information