HUGE

Gene/Protein Characteristic Table for KIAA0399

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01083
Accession No. :	AB007859
Description :	Zinc finger ZZ-type and EF-hand domain-containing protein 1.
HUGO Gene Name :	zinc finger, ZZ-type with EF-hand domain 1 (ZZEF1)
Clone Name :	hg00651s2 [Vector Info]
Flexi ORF Clone :	pF1KA0399
Source :	Human adult brain
Note :	We replaced hg00651 and hg00651s1, former representative clones for KIAA0399 with hg00651s2. (2002/5/10,2005/08/06)

Features of the cloned DNA sequence	Description

Length: 11394 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2444 bp

Genome contig ID gi51511734r_3754489
PolyA signal sequence
(AATATA,-31)
+----*----+----*----+----*----+----
AAGAAATATATTGTACAAATTCTATATAATAAGGT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACGCCTGTGTGTGGACTGGTCTCTCCCTCCTCCCTCCGCCCCGCCGTGTC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 17 r 3854489 3993002 55 99.7 Perfect prediction

Features of the protein sequence	Description

Length: 2982 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_511813	99.6	zinc finger, ZZ...	Pan troglodytes
O43149	100.0	Zinc finger ZZ-...	Homo sapiens
NP_055928	99.9	zinc finger, ZZ...	Homo sapiens
XP_001117691	97.8	similar to zinc...	Macaca mulatta
XP_869342	91.8	similar to Zinc...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D38548	1.5e-08	42.4	KIAA0076
AB014608	7.9e-08	40.9	KIAA0708

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR002048 136 164 PF00036 Calcium-binding EF-hand

IPR004939 253 418 PF03256 Anaphase-promoting complex subunit 10

IPR000433 1798 1846 PF00569 Zinc finger

IPR000433 1847 1891 PF00569 Zinc finger

HMMSmart IPR002048 136 164 SM00054 Calcium-binding EF-hand

IPR000433 1798 1846 SM00291 Zinc finger

IPR000433 1847 1891 SM00291 Zinc finger

ProfileScan IPR002048 132 167 PS50222 Calcium-binding EF-hand

IPR000433 1798 1848 PS50135 Zinc finger

IPR000433 1847 1893 PS50135 Zinc finger

ScanRegExp IPR000433 1853 1879 PS01357 Zinc finger

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002048	136	164	PF00036	Calcium-binding EF-hand
IPR004939	253	418	PF03256	Anaphase-promoting complex subunit 10
IPR000433	1798	1846	PF00569	Zinc finger
IPR000433	1847	1891	PF00569	Zinc finger
HMMSmart	IPR002048	136	164	SM00054	Calcium-binding EF-hand
IPR000433	1798	1846	SM00291	Zinc finger
IPR000433	1847	1891	SM00291	Zinc finger
ProfileScan	IPR002048	132	167	PS50222	Calcium-binding EF-hand
IPR000433	1798	1848	PS50135	Zinc finger
IPR000433	1847	1893	PS50135	Zinc finger
ScanRegExp	IPR000433	1853	1879	PS01357	Zinc finger

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 2157 TLGLLGQLIIRLLPAEVDAAVIK 2179 SECONDARY 23

2 2262 NIFTLLVLVGFPQVLCVGTRCVY 2284 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2157	TLGLLGQLIIRLLPAEVDAAVIK	2179	SECONDARY	23
2	2262	NIFTLLVLVGFPQVLCVGTRCVY	2284	PRIMARY	23

Expression profile	Description

: RT-PCR Description

Experimental conditions

Primer_f
: GAAAATACGTCACTCCTCTCG

Primer_r
: AAGTCCCGCAGAGCAAAGGTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RH mapping information	Description

Chromosome No.

: 17

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GAAAATACGTCACTCCTCTCG
Primer_r	: AAGTCCCGCAGAGCAAAGGTG
PCR product length	: 115 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	2444 bp
Genome contig ID	gi51511734r_3754489
PolyA signal sequence (AATATA,-31)	+--------+--------+----*----+---- AAGAAATATATTGTACAAATTCTATATAATAAGGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACGCCTGTGTGTGGACTGGTCTCTCCCTCCTCCCTCCGCCCCGCCGTGTC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	17	r	3854489	3993002	55	99.7	Perfect prediction

Gene/Protein Characteristic Table for KIAA0399

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information