HUGE

Gene/Protein Characteristic Table for KIAA0702

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB014602
Description :	Sodium/potassium/calcium exchanger 1.
HUGO Gene Name :	solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1)
Clone Name :	hg01814 [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5295 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
NO NO

Warning for coding interruption:
YES YES

Integrity of 3' end

Length of 3'UTR 1041 bp

Genome contig ID gi51511731f_63590757
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
CATTATACATAGAATAAAAGGGCATTTATATTGTT
Flanking genome sequence
(141566 - 141615)
----+----*----+----*----+----*----+----*----+----*
AGTTTCTGCAAAGCAACAAAATATCTTCCCTTCATTCATCATAGCCAGAT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 15 f 63690757 63732321 8 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 988 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW77741	100.0	solute carrier ...	Homo sapiens
XP_001174578	98.6	solute carrier ...	Pan troglodytes
EAW77739	99.9	solute carrier ...	Homo sapiens
AAB97832	99.8	retinal rod Na+...	Homo sapiens
BAG64922	99.7	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR004837 471 603 PF01699 Sodium/calcium exchanger membrane region

HMMTigr IPR004817 4 984 TIGR00927 K+-dependent Na+/Ca+ exchanger

IPR004481 460 984 TIGR00367 K+-dependent Na+/Ca+ exchanger related-protein

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004837	471	603	PF01699	Sodium/calcium exchanger membrane region
HMMTigr	IPR004817	4	984	TIGR00927	K+-dependent Na+/Ca+ exchanger
IPR004481	460	984	TIGR00367	K+-dependent Na+/Ca+ exchanger related-protein

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 23 LHWSRLLFLLGMLIIGSTYQHL 44 SECONDARY 22

2 457 WVVLHVFGMMYVFVALAIVCDEY 479 PRIMARY 23

3 526 VGIGTIVGSAVFNILFVIGTCSL 548 PRIMARY 23

4 574 MLILFFLDSLIAWWESLLLLLAY 596 PRIMARY 23

5 894 AIYLFLLPIVFPLWLTVPDV 913 PRIMARY 20

6 922 FVFTFLGSIMWIAMFSYLMVWWA 944 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	23	LHWSRLLFLLGMLIIGSTYQHL	44	SECONDARY	22
2	457	WVVLHVFGMMYVFVALAIVCDEY	479	PRIMARY	23
3	526	VGIGTIVGSAVFNILFVIGTCSL	548	PRIMARY	23
4	574	MLILFFLDSLIAWWESLLLLLAY	596	PRIMARY	23
5	894	AIYLFLLPIVFPLWLTVPDV	913	PRIMARY	20
6	922	FVFTFLGSIMWIAMFSYLMVWWA	944	PRIMARY	23

Expression profile	Description

: RT-PCR Description
: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCTGCCTGGAATACTGAACTG

Primer_r
: ACTAAGAGGCTGGAGGTGAAG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 15

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: AACCAGTGAACAGAGCCAATG
Primer_r	: TGTGATTGTCGCTCGAAAAGG
PCR product length	: 155 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	NO	NO
Warning for coding interruption:	YES	YES

Length of 3'UTR	1041 bp
Genome contig ID	gi51511731f_63590757
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- CATTATACATAGAATAAAAGGGCATTTATATTGTT
Flanking genome sequence (141566 - 141615)	----+--------+--------+--------+--------+----* AGTTTCTGCAAAGCAACAAAATATCTTCCCTTCATTCATCATAGCCAGAT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	15	f	63690757	63732321	8	99.6	Perfect prediction

Primer_f	: TCTGCCTGGAATACTGAACTG
Primer_r	: ACTAAGAGGCTGGAGGTGAAG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0702

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information