HUGE

Gene/Protein Characteristic Table for KIAA0735

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00617
Accession No. :	AB018278
Description :	Synaptic vesicle glycoprotein 2B.
HUGO Gene Name :	synaptic vesicle glycoprotein 2B (SV2B)
Clone Name :	hj00943 [Vector Info]
Flexi ORF Clone :	pF1KSDA0735
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4948 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2732 bp

Genome contig ID gi51511731f_89470349
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
TGATCTTTTGTTTTATTAAAAATAATTAGTGAAAG
Flanking genome sequence
(169171 - 169220)
----+----*----+----*----+----*----+----*----+----*
AGGTGTGCCTATCTGTGAAGTTTGTAGTACATCATCCTGAGGTCATGTAA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 15 f 89570334 89639518 12 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 707 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001099688	99.6	similar to syna...	Macaca mulatta
Q7L1I2	100.0	Synaptic vesicl...	Homo sapiens
CAG33367	99.9	SV2B [Homo sapi...	Homo sapiens
XP_545841	95.2	similar to syna...	Canis lupus fam...
XP_523160	99.3	synaptic vesicl...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB018279	7.1e-164	62.4	KIAA0736
AB028977	1.6e-119	62.3	KIAA1054

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR011701 139 674 PF07690 Major facilitator superfamily MFS_1

HMMTigr IPR005988 1 707 TIGR01299 Synaptic vesicle protein SV2

ProfileScan IPR007114 135 702 PS50850 Major facilitator superfamily

ScanRegExp IPR005829 189 205 PS00216 Sugar transporter superfamily

IPR005829 231 256 PS00217 Sugar transporter superfamily

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011701	139	674	PF07690	Major facilitator superfamily MFS_1
HMMTigr	IPR005988	1	707	TIGR01299	Synaptic vesicle protein SV2
ProfileScan	IPR007114	135	702	PS50850	Major facilitator superfamily
ScanRegExp	IPR005829	189	205	PS00216	Sugar transporter superfamily
IPR005829	231	256	PS00217	Sugar transporter superfamily

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 134 ILFFVLGLALMADGVEVFVVSFA 156 PRIMARY 23

2 173 MLGMIVYLGMMAGAFILGGLADK 195 PRIMARY 23

3 229 RLISGIGIGGALPIVFAYFSEFL 251 PRIMARY 23

4 261 SWLGIFWMTGGLYASAMAWSIIP 283 SECONDARY 23

5 300 WRVFVIVCALPCTVSMVALKFMP 322 PRIMARY 23

6 416 ILAVVWFAMAFSYYGLTVWFPDM 438 PRIMARY 23

7 561 LIYLVSFLGSLSVLPGNIISALL 583 SECONDARY 23

8 591 KMIGGSMLISAVCCFFLFF 609 PRIMARY 19

9 619 WQCLFCGTSIAAWNALDVITVEL 641 SECONDARY 23

10 649 TAFGILNGLCKFGAILGNTIFAS 671 SECONDARY 23

11 681 ILLAAASLVGGGLIALRLPETRE 703 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	134	ILFFVLGLALMADGVEVFVVSFA	156	PRIMARY	23
2	173	MLGMIVYLGMMAGAFILGGLADK	195	PRIMARY	23
3	229	RLISGIGIGGALPIVFAYFSEFL	251	PRIMARY	23
4	261	SWLGIFWMTGGLYASAMAWSIIP	283	SECONDARY	23
5	300	WRVFVIVCALPCTVSMVALKFMP	322	PRIMARY	23
6	416	ILAVVWFAMAFSYYGLTVWFPDM	438	PRIMARY	23
7	561	LIYLVSFLGSLSVLPGNIISALL	583	SECONDARY	23
8	591	KMIGGSMLISAVCCFFLFF	609	PRIMARY	19
9	619	WQCLFCGTSIAAWNALDVITVEL	641	SECONDARY	23
10	649	TAFGILNGLCKFGAILGNTIFAS	671	SECONDARY	23
11	681	ILLAAASLVGGGLIALRLPETRE	703	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCTTAAATGCCGTCACTCCTG

Primer_r
: AGTGAAAAAGAAGTGGATGGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 15

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TTACCCTTCCACACAATAGCC
Primer_r	: AAAACTACAACACGTCACAGC
PCR product length	: 110 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	2732 bp
Genome contig ID	gi51511731f_89470349
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- TGATCTTTTGTTTTATTAAAAATAATTAGTGAAAG
Flanking genome sequence (169171 - 169220)	----+--------+--------+--------+--------+----* AGGTGTGCCTATCTGTGAAGTTTGTAGTACATCATCCTGAGGTCATGTAA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	15	f	89570334	89639518	12	99.4	Perfect prediction

Primer_f	: TCTTAAATGCCGTCACTCCTG
Primer_r	: AGTGAAAAAGAAGTGGATGGC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0735

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information