HUGE

Gene/Protein Characteristic Table for KIAA0778

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00634
Accession No. :	AB018321
Description :	Sodium/potassium-transporting ATPase subunit alpha-2 precursor.
HUGO Gene Name :
Clone Name :	fh12074 [Vector Info]
Flexi ORF Clone :	pF1KSDA0778
Source :	Human fetal brain
Note :	We replaced hk05292, former representative clones for KIAA0778 with fh12074. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5411 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2259 bp

Genome contig ID gi89161185f_158252187
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
TGTACTTGAAAATAATAAAGTGGCATTTCTTTAAG
Flanking genome sequence
(127811 - 127860)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAGAAATCTCTTCCATAATTCAGATTTCTACACTTTATACTTG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 f 158352187 158379996 23 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 1049 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001115318	99.7	Na+/K+ -ATPase ...	Macaca mulatta
P50993	100.0	Sodium/potassiu...	Homo sapiens
BAG36951	99.9	unnamed protein...	Homo sapiens
Q5RCD8	99.5	Sodium/potassiu...	Pongo abelii
XP_001929066	99.1	Na,K-ATPase alp...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037768	6.1e-26	32.2	KIAA1347
AB014603	1.1e-25	32.8	KIAA0703
AB058728	0.00012	22.9	KIAA1825

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR006069 130 144 PR00121 ATPase

IPR006069 157 177 PR00121 ATPase

IPR001757 241 255 PR00119 ATPase

IPR006069 321 343 PR00121 ATPase

IPR006069 394 415 PR00121 ATPase

IPR001757 401 415 PR00119 ATPase

IPR006069 531 549 PR00121 ATPase

IPR001757 613 624 PR00119 ATPase

IPR001757 635 645 PR00119 ATPase

IPR001757 741 760 PR00119 ATPase

IPR001757 765 777 PR00119 ATPase

IPR006069 813 834 PR00121 ATPase

IPR006069 880 900 PR00121 ATPase

IPR006069 942 962 PR00121 ATPase

IPR006069 976 1000 PR00121 ATPase

HMMPfam IPR004014 60 143 PF00690 ATPase

IPR008250 162 393 PF00122 E1-E2 ATPase-associated region

IPR005834 397 764 PF00702 Haloacid dehalogenase-like hydrolase

IPR006068 860 1038 PF00689 ATPase

HMMTigr IPR005775 54 1049 TIGR01106 ATPase

IPR001757 162 254 TIGR01494 ATPase

IPR001757 306 431 TIGR01494 ATPase

IPR001757 610 660 TIGR01494 ATPase

IPR001757 715 834 TIGR01494 ATPase

ScanRegExp IPR001757 403 409 PS00154 ATPase

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR006069	130	144	PR00121	ATPase
IPR006069	157	177	PR00121	ATPase
IPR001757	241	255	PR00119	ATPase
IPR006069	321	343	PR00121	ATPase
IPR006069	394	415	PR00121	ATPase
IPR001757	401	415	PR00119	ATPase
IPR006069	531	549	PR00121	ATPase
IPR001757	613	624	PR00119	ATPase
IPR001757	635	645	PR00119	ATPase
IPR001757	741	760	PR00119	ATPase
IPR001757	765	777	PR00119	ATPase
IPR006069	813	834	PR00121	ATPase
IPR006069	880	900	PR00121	ATPase
IPR006069	942	962	PR00121	ATPase
IPR006069	976	1000	PR00121	ATPase
HMMPfam	IPR004014	60	143	PF00690	ATPase
IPR008250	162	393	PF00122	E1-E2 ATPase-associated region
IPR005834	397	764	PF00702	Haloacid dehalogenase-like hydrolase
IPR006068	860	1038	PF00689	ATPase
HMMTigr	IPR005775	54	1049	TIGR01106	ATPase
IPR001757	162	254	TIGR01494	ATPase
IPR001757	306	431	TIGR01494	ATPase
IPR001757	610	660	TIGR01494	ATPase
IPR001757	715	834	TIGR01494	ATPase
ScanRegExp	IPR001757	403	409	PS00154	ATPase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 2 RLSQTGWCGLGILLVTSPAKVPQ 24 SECONDARY 23

2 125 GGFSILLWIGAILCFLAYGIQAA 147 PRIMARY 23

3 157 LYLGVVLAAVVIVTGCFSYYQ 177 PRIMARY 21

4 319 IQLITGVAVFLGVSFFVLSLILG 341 PRIMARY 23

5 351 LIGIIVANVPEGLLATVTVCLTL 373 PRIMARY 23

6 821 IANIPLPLGTVTILCIDLGTDMV 843 SECONDARY 23

7 941 EFTCHTAFFASIVVVQWADLIIC 963 SECONDARY 23

8 981 IFGLLEETALAAFLSYCPGMGVA 1003 SECONDARY 23

9 1009 LKVTWWFCAFPYSLLIFIYDEV 1030 PRIMARY 22

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	RLSQTGWCGLGILLVTSPAKVPQ	24	SECONDARY	23
2	125	GGFSILLWIGAILCFLAYGIQAA	147	PRIMARY	23
3	157	LYLGVVLAAVVIVTGCFSYYQ	177	PRIMARY	21
4	319	IQLITGVAVFLGVSFFVLSLILG	341	PRIMARY	23
5	351	LIGIIVANVPEGLLATVTVCLTL	373	PRIMARY	23
6	821	IANIPLPLGTVTILCIDLGTDMV	843	SECONDARY	23
7	941	EFTCHTAFFASIVVVQWADLIIC	963	SECONDARY	23
8	981	IFGLLEETALAAFLSYCPGMGVA	1003	SECONDARY	23
9	1009	LKVTWWFCAFPYSLLIFIYDEV	1030	PRIMARY	22

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TGGAGATTACTAACTGTGGAC

Primer_r
: GATAATTAGCCCATGCACTCG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: UniGene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	2259 bp
Genome contig ID	gi89161185f_158252187
PolyA signal sequence (AATAAA,-22)	+--------+--------+----*----+---- TGTACTTGAAAATAATAAAGTGGCATTTCTTTAAG
Flanking genome sequence (127811 - 127860)	----+--------+--------+--------+--------+----* AAAAAAAAAGAAATCTCTTCCATAATTCAGATTTCTACACTTTATACTTG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	f	158352187	158379996	23	99.5	Perfect prediction

Primer_f	: TGGAGATTACTAACTGTGGAC
Primer_r	: GATAATTAGCCCATGCACTCG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0778

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information