HUGE

Gene/Protein Characteristic Table for KIAA0886

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00673
Accession No. :	AB020693
Description :	Reticulon-4.
HUGO Gene Name :	reticulon 4 (RTN4)
Clone Name :	hk07722 [Vector Info]
Flexi ORF Clone :	pF1KSDA0886
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4053 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 340 bp

Genome contig ID gi89161199r_54953452
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
CGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAG
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 r 55053452 55131074 9 99.8 Perfect prediction

Features of the protein sequence	Description

Length: 1233 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9NQC3	100.0	Reticulon-4; Ne...	Homo sapiens
CAB99248	99.9	Nogo-A protein ...	Homo sapiens
XP_001112090	95.7	reticulon 4 [Ma...	Macaca mulatta
EAX00129	98.4	reticulon 4, is...	Homo sapiens
AAM64244	100.0	RNT4 [Homo sapi...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR003388 1046 1231 PF02453 Reticulon

ProfileScan IPR003388 1046 1233 PS50845 Reticulon

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003388	1046	1231	PF02453	Reticulon
ProfileScan	IPR003388	1046	1233	PS50845	Reticulon

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 1047 VDLLYWRDIKKTGVVFGASLFLL 1069 SECONDARY 23

2 1075 FSIVSVTAYIALALLSVTISFRI 1097 PRIMARY 23

3 1150 LFLVDDLVDSLKFAVLMWVFTYV 1172 PRIMARY 23

4 1176 FNGLTLLILALISLFSVPVIYER 1198 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1047	VDLLYWRDIKKTGVVFGASLFLL	1069	SECONDARY	23
2	1075	FSIVSVTAYIALALLSVTISFRI	1097	PRIMARY	23
3	1150	LFLVDDLVDSLKFAVLMWVFTYV	1172	PRIMARY	23
4	1176	FNGLTLLILALISLFSVPVIYER	1198	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GATTATACGGGGGAGGGTCAG

Primer_r
: ACACATGGCAGTCAAGACAGG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GATTATACGGGGGAGGGTCAG
Primer_r	: ACACATGGCAGTCAAGACAGG
PCR product length	: 131 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	340 bp
Genome contig ID	gi89161199r_54953452
PolyA signal sequence (None)	+--------+--------+----*----+---- CGCATCTTGGCAAGTTGCAGAGATGGTGGAGCTAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAGCCCTTTTCAGTTTGTGCACTGTGTATGGTCCGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	r	55053452	55131074	9	99.8	Perfect prediction

Gene/Protein Characteristic Table for KIAA0886

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information