HUGE

Gene/Protein Characteristic Table for KIAA1145

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00187
Accession No. :	AB032971
Description :	Transmembrane and coiled-coil domains protein 3.
HUGO Gene Name :	transmembrane and coiled-coil domain family 3 (TMCC3)
Clone Name :	hk10430 [Vector Info]
Flexi ORF Clone :	pF1KA1145
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4361 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2866 bp

Genome contig ID gi89161190r_93386476
PolyA signal sequence
(ATTAAA,-23)
+----*----+----*----+----*----+----
AGAAATATTACAATTAAAAATAGTGATGTAGCTGT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AACATATGCTGGAATTGGATATTTAATTTATGTTTGTGTCAACTATAATC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 12 r 93486476 93533968 4 99.1 Perfect prediction

Features of the protein sequence	Description

Length: 467 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
EAW97510	3.6e-157	97.6	transmembrane a...	Homo sapiens
Q9ULS5	1.5e-156	97.4	Transmembrane a...	Homo sapiens
CAH90408	6.4e-156	96.6	hypothetical pr...	Pongo abelii
EAW97512	4.9e-155	100.0	transmembrane a...	Homo sapiens
EDM16881	1.5e-149	92.7	transmembrane a...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007950	5.3e-61	55.7	KIAA0481
AB018322	1.8e-34	53.3	KIAA0779

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

None - - - - -

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 399 VLLGRCINVILAFMTVILVCVST 421 PRIMARY 23

2 433 RCHILGTFFAVTLLAIFCKNWDH 455 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	399	VLLGRCINVILAFMTVILVCVST	421	PRIMARY	23
2	433	RCHILGTFFAVTLLAIFCKNWDH	455	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CGTAGGCTTCATTTTCACACC

Primer_r
: CTTCTCTACTTCTGTCACTGG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 12

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	2866 bp
Genome contig ID	gi89161190r_93386476
PolyA signal sequence (ATTAAA,-23)	+--------+--------+----*----+---- AGAAATATTACAATTAAAAATAGTGATGTAGCTGT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AACATATGCTGGAATTGGATATTTAATTTATGTTTGTGTCAACTATAATC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	12	r	93486476	93533968	4	99.1	Perfect prediction

Primer_f	: CGTAGGCTTCATTTTCACACC
Primer_r	: CTTCTCTACTTCTGTCACTGG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1145

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information