HUGE

Gene/Protein Characteristic Table for KIAA1169

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00754
Accession No. :	AB032995
Description :	Two-pore calcium channel protein 1.
HUGO Gene Name :	two pore segment channel 1 (TPCN1)
Clone Name :	hh10053 [Vector Info]
Flexi ORF Clone :	pF1KSDA1169
Source :	Human adult brain
Note :	We replaced hg02358, former representative clones for KIAA1169 with hh10053. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 5238 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 2506 bp

Genome contig ID gi89161190f_112043751
PolyA signal sequence
(ACTAAA,-22)
+----*----+----*----+----*----+----
TTTCTAGCACATTACTAAAAGAGCCTCTGCTTTGT
Flanking genome sequence
(177021 - 177070)
----+----*----+----*----+----*----+----*----+----*
AAACATGGCTGCTGCCTCTTGACTTCTGTGGCTTTGGGTATTGTCGGGAG

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 12 f 112143659 112220770 28 99.5 Perfect prediction

Features of the protein sequence	Description

Length: 857 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
NP_001137291	100.0	two pore segmen...	Homo sapiens
EAW98047	100.0	two pore segmen...	Homo sapiens
XP_001111625	98.7	similar to two ...	Macaca mulatta
Q9ULQ1	100.0	Two pore calciu...	Homo sapiens
BAG52729	99.9	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037777	1.5e-07	25.3	KIAA1356
AB032946	5.6e-07	22.4	KIAA1120
AB032949	1.8e-06	21.9	KIAA1123

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR005821 183 360 PF00520 Ion transport

IPR005821 518 727 PF00520 Ion transport

ScanRegExp IPR001220 357 363 PS00307 Legume lectin

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005821	183	360	PF00520	Ion transport
IPR005821	518	727	PF00520	Ion transport
ScanRegExp	IPR001220	357	363	PS00307	Legume lectin

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 148 LFYLMELATALLLLLLSLCEAPA 170 PRIMARY 23

2 176 LGIYVHATLELFALMVVVFELCM 198 PRIMARY 23

3 215 MVKTSVLVVQFVEAIVVLVRQMS 237 SECONDARY 23

4 270 LPPFMDILLLLLFFMIIFAILGF 292 PRIMARY 23

5 303 YFSTLENSIVSLFVLLTTANFPD 325 SECONDARY 23

6 339 VFFIVYLSIELYFIMNLLLAVVF 361 PRIMARY 23

7 487 FMYLVVAVNGVWILVETFMLKGG 509 PRIMARY 23

8 517 PWSYLVFLTIYGVELFLKVAGLG 539 PRIMARY 23

9 560 FLGLLALALNMEPFYFIVVLRPL 582 PRIMARY 23

10 612 GLTLLIFYYSFAIVGMEFFCGIV 634 PRIMARY 23

11 710 MTFYIVTMVVMTIIVAFILEAFV 732 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	148	LFYLMELATALLLLLLSLCEAPA	170	PRIMARY	23
2	176	LGIYVHATLELFALMVVVFELCM	198	PRIMARY	23
3	215	MVKTSVLVVQFVEAIVVLVRQMS	237	SECONDARY	23
4	270	LPPFMDILLLLLFFMIIFAILGF	292	PRIMARY	23
5	303	YFSTLENSIVSLFVLLTTANFPD	325	SECONDARY	23
6	339	VFFIVYLSIELYFIMNLLLAVVF	361	PRIMARY	23
7	487	FMYLVVAVNGVWILVETFMLKGG	509	PRIMARY	23
8	517	PWSYLVFLTIYGVELFLKVAGLG	539	PRIMARY	23
9	560	FLGLLALALNMEPFYFIVVLRPL	582	PRIMARY	23
10	612	GLTLLIFYYSFAIVGMEFFCGIV	634	PRIMARY	23
11	710	MTFYIVTMVVMTIIVAFILEAFV	732	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CCAGAGCGAGTTAATGTGTCC

Primer_r
: AGCTGTCCCTAACCTCAATGA

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 12

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	2506 bp
Genome contig ID	gi89161190f_112043751
PolyA signal sequence (ACTAAA,-22)	+--------+--------+----*----+---- TTTCTAGCACATTACTAAAAGAGCCTCTGCTTTGT
Flanking genome sequence (177021 - 177070)	----+--------+--------+--------+--------+----* AAACATGGCTGCTGCCTCTTGACTTCTGTGGCTTTGGGTATTGTCGGGAG

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	12	f	112143659	112220770	28	99.5	Perfect prediction

Primer_f	: CCAGAGCGAGTTAATGTGTCC
Primer_r	: AGCTGTCCCTAACCTCAATGA
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1169

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information