HUGE

Gene/Protein Characteristic Table for KIAA1180

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK01154
Accession No. :	AB033006
Description :	Protein NDRG4.
HUGO Gene Name :	NDRG family member 4 (NDRG4)
Clone Name :	hf00665as1 [Vector Info]
Flexi ORF Clone :	pF1KA1180
Source :	Human adult brain
Note :	We replaced hf00665a, former representative clones for KIAA1180 with hf00665as1. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 2994 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1843 bp

Genome contig ID gi51511732f_56991562
PolyA signal sequence
(AATAAA,-18)
+----*----+----*----+----*----+----
AACTGTGCTGAAAAACTAATAAAGAACCTAAGAAG
Flanking genome sequence
(113258 - 113307)
----+----*----+----*----+----*----+----*----+----*
AATTCCAGTGTGGTGATGCCATGCCCATCATGGGAGGCTTTTGGAGAAAC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 16 f 57091562 57104818 15 99.3 Perfect prediction

Features of the protein sequence	Description

Length: 360 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9ULP0	1.5e-148	100.0	Protein NDRG4; ...	Homo sapiens
Q8BTG7	7.5e-146	97.7	Protein NDRG4; ...	Mus musculus
EDL87274	6.3e-145	97.2	N-myc downstrea...	Rattus norvegicus
Q9Z2L9	1.2e-144	96.9	Protein NDRG4; ...	Rattus norvegicus
AAO65544	2.5e-140	96.3	brain and heart...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB033074	6e-79	62.0	KIAA1248

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR004142 16 298 PF03096 Ndr

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004142	16	298	PF03096	Ndr

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 120 GVGAGAYVLAKFALIFPDLVEGL 142 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	120	GVGAGAYVLAKFALIFPDLVEGL	142	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GGACGCATCACTAAGGAAGAG

Primer_r
: AAGCATACAACGCAGTCCTGG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 16

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	1843 bp
Genome contig ID	gi51511732f_56991562
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- AACTGTGCTGAAAAACTAATAAAGAACCTAAGAAG
Flanking genome sequence (113258 - 113307)	----+--------+--------+--------+--------+----* AATTCCAGTGTGGTGATGCCATGCCCATCATGGGAGGCTTTTGGAGAAAC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	16	f	57091562	57104818	15	99.3	Perfect prediction

Primer_f	: GGACGCATCACTAAGGAAGAG
Primer_r	: AAGCATACAACGCAGTCCTGG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1180

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information