HUGE

Gene/Protein Characteristic Table for KIAA1282

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB033108
Description :	Potassium voltage-gated channel subfamily H member 3.
HUGO Gene Name :
Clone Name :	hh04440b [Vector Info]
Flexi ORF Clone :	pF1KA1282
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 3723 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
NO NO

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 341 bp

Genome contig ID gi89161190f_48119207
PolyA signal sequence
(AATAAA,-8)
+----*----+----*----+----*----+----
CCCCAAATTTTTATATTAAAAAAAAAAAATAAAAT
Flanking genome sequence
(119139 - 119188)
----+----*----+----*----+----*----+----*----+----*
AAACTACTTTGGAACCTGGTGCTTTTTATTTACAAAAGAAAAACAATAAA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 12 f 48219207 48238344 15 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 1117 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9ULD8	100.0	Potassium volta...	Homo sapiens
EAW58074	99.9	potassium volta...	Homo sapiens
XP_509046	99.7	potassium volta...	Pan troglodytes
XP_001109598	99.4	potassium volta...	Macaca mulatta
XP_001504252	96.5	similar to Pota...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040968	0.00018	25.1	KIAA1535

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR003950 135 144 PR01465 ELK potassium channel

IPR003950 148 156 PR01465 ELK potassium channel

IPR003938 253 262 PR01463 EAG/ELK/ERG potassium channel

IPR003938 271 278 PR01463 EAG/ELK/ERG potassium channel

IPR003938 299 309 PR01463 EAG/ELK/ERG potassium channel

IPR003938 310 319 PR01463 EAG/ELK/ERG potassium channel

IPR003950 320 332 PR01465 ELK potassium channel

IPR003950 387 395 PR01465 ELK potassium channel

IPR003938 403 413 PR01463 EAG/ELK/ERG potassium channel

IPR003938 486 503 PR01463 EAG/ELK/ERG potassium channel

IPR003938 512 523 PR01463 EAG/ELK/ERG potassium channel

IPR003950 523 530 PR01465 ELK potassium channel

IPR003938 530 539 PR01463 EAG/ELK/ERG potassium channel

IPR003938 580 587 PR01463 EAG/ELK/ERG potassium channel

IPR003950 641 651 PR01465 ELK potassium channel

IPR003938 696 704 PR01463 EAG/ELK/ERG potassium channel

HMMPfam IPR013655 76 170 PF08447 PAS fold-3

IPR005821 297 534 PF00520 Ion transport

IPR000595 634 721 PF00027 Cyclic nucleotide-binding

HMMSmart IPR001610 128 170 SM00086 PAC motif

IPR000595 616 731 SM00100 Cyclic nucleotide-binding

HMMTigr IPR000014 50 177 TIGR00229 PAS

ProfileScan IPR000014 76 124 PS50112 PAS

IPR000700 127 179 PS50113 PAS-associated

IPR000595 616 680 PS50042 Cyclic nucleotide-binding

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003950	135	144	PR01465	ELK potassium channel
IPR003950	148	156	PR01465	ELK potassium channel
IPR003938	253	262	PR01463	EAG/ELK/ERG potassium channel
IPR003938	271	278	PR01463	EAG/ELK/ERG potassium channel
IPR003938	299	309	PR01463	EAG/ELK/ERG potassium channel
IPR003938	310	319	PR01463	EAG/ELK/ERG potassium channel
IPR003950	320	332	PR01465	ELK potassium channel
IPR003950	387	395	PR01465	ELK potassium channel
IPR003938	403	413	PR01463	EAG/ELK/ERG potassium channel
IPR003938	486	503	PR01463	EAG/ELK/ERG potassium channel
IPR003938	512	523	PR01463	EAG/ELK/ERG potassium channel
IPR003950	523	530	PR01465	ELK potassium channel
IPR003938	530	539	PR01463	EAG/ELK/ERG potassium channel
IPR003938	580	587	PR01463	EAG/ELK/ERG potassium channel
IPR003950	641	651	PR01465	ELK potassium channel
IPR003938	696	704	PR01463	EAG/ELK/ERG potassium channel
HMMPfam	IPR013655	76	170	PF08447	PAS fold-3
IPR005821	297	534	PF00520	Ion transport
IPR000595	634	721	PF00027	Cyclic nucleotide-binding
HMMSmart	IPR001610	128	170	SM00086	PAC motif
IPR000595	616	731	SM00100	Cyclic nucleotide-binding
HMMTigr	IPR000014	50	177	TIGR00229	PAS
ProfileScan	IPR000014	76	124	PS50112	PAS
IPR000700	127	179	PS50113	PAS-associated
IPR000595	616	680	PS50042	Cyclic nucleotide-binding

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 263 GFILLATLYVAVTVPYSVCVSTA 285 PRIMARY 23

2 337 VTTWFLLDVIAALPFDLLHAFKV 359 PRIMARY 23

3 391 AVVLTLLMAVFALLAHWVACVWF 413 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	263	GFILLATLYVAVTVPYSVCVSTA	285	PRIMARY	23
2	337	VTTWFLLDVIAALPFDLLHAFKV	359	PRIMARY	23
3	391	AVVLTLLMAVFALLAHWVACVWF	413	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TGCCTGCTCCTTCCTTTATGG

Primer_r
: TTTCGCTGATGTCCTTGTGAG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 12

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TGCCTGCTCCTTCCTTTATGG
Primer_r	: TTTCGCTGATGTCCTTGTGAG
PCR product length	: 212 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	NO	NO
Warning for coding interruption:	YES	NO

Length of 3'UTR	341 bp
Genome contig ID	gi89161190f_48119207
PolyA signal sequence (AATAAA,-8)	+--------+--------+----*----+---- CCCCAAATTTTTATATTAAAAAAAAAAAATAAAAT
Flanking genome sequence (119139 - 119188)	----+--------+--------+--------+--------+----* AAACTACTTTGGAACCTGGTGCTTTTTATTTACAAAAGAAAAACAATAAA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	12	f	48219207	48238344	15	100.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA1282

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information