HUGE

Gene/Protein Characteristic Table for KIAA1532

Link to : GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB040965
Description :	Signal peptide peptidase-like 2B.
HUGO Gene Name :
Clone Name :	fg04455 [Vector Info]
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 6722 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 1592 bp

Genome contig ID gi42406306f_2179680
PolyA signal sequence
(ATTAAA,-21)
+----*----+----*----+----*----+----
CATTCCGTCTGATCATTAAACACAGCTTTTGATAC
Flanking genome sequence
(126121 - 126170)
----+----*----+----*----+----*----+----*----+----*
ATATGGCCCGGCCTCTGCCTCCGCAGTGCACGGCTTGGGGCATCTGCTCT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 19 f 2279680 2305799 15 99.9 Perfect prediction

Features of the protein sequence	Description

Length: 601 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q8TCT7	3.5e-193	100.0	Signal peptide ...	Homo sapiens
CAD13134	5.4e-185	100.0	SPPL2b protein ...	Homo sapiens
AAH28391	5.9e-162	100.0	Signal peptide ...	Homo sapiens
XP_580381	3.7e-158	83.8	signal peptide ...	Bos taurus
XP_542189	6.1e-158	83.9	similar to sign...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR003137 64 164 PF02225 Protease-associated PA

IPR007369 222 512 PF04258 Peptidase A22B

HMMSmart IPR006639 226 501 SM00730 Peptidase A22

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003137	64	164	PF02225	Protease-associated PA
IPR007369	222	512	PF04258	Peptidase A22B
HMMSmart	IPR006639	226	501	SM00730	Peptidase A22

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 12 AAVAAALARLLAAFLLLAAQVAC 34 PRIMARY 23

2 179 VLDYNMVIIFIMAVGTVAIGGYW 201 PRIMARY 23

3 227 AVDVTPVMTCVFVVMCCSMLVLL 249 PRIMARY 23

4 255 LLVYVVIGIFCLASATGLYSCLA 277 PRIMARY 23

5 303 QARMLLLALFCVAVSVVWGVFRN 325 PRIMARY 23

6 329 WAWVLQDALGIAFCLYMLKTIRL 351 PRIMARY 23

7 355 KACTLLLLVLFLYDIFFVFITPF 377 PRIMARY 23

8 424 SLLGFGDILVPGLLVAYCHRFD 445 SECONDARY 22

9 454 YFVACTIAYGVGLLVTFVALALM 476 PRIMARY 23

10 479 GQPALLYLVPCTLVTSCAVALW 500 PRIMARY 22

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	12	AAVAAALARLLAAFLLLAAQVAC	34	PRIMARY	23
2	179	VLDYNMVIIFIMAVGTVAIGGYW	201	PRIMARY	23
3	227	AVDVTPVMTCVFVVMCCSMLVLL	249	PRIMARY	23
4	255	LLVYVVIGIFCLASATGLYSCLA	277	PRIMARY	23
5	303	QARMLLLALFCVAVSVVWGVFRN	325	PRIMARY	23
6	329	WAWVLQDALGIAFCLYMLKTIRL	351	PRIMARY	23
7	355	KACTLLLLVLFLYDIFFVFITPF	377	PRIMARY	23
8	424	SLLGFGDILVPGLLVAYCHRFD	445	SECONDARY	22
9	454	YFVACTIAYGVGLLVTFVALALM	476	PRIMARY	23
10	479	GQPALLYLVPCTLVTSCAVALW	500	PRIMARY	22

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CTGGTGCTGTTCCTCTACGAC

Primer_r
: TGTACCTGGATGTCAAACCTG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 19

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	1592 bp
Genome contig ID	gi42406306f_2179680
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- CATTCCGTCTGATCATTAAACACAGCTTTTGATAC
Flanking genome sequence (126121 - 126170)	----+--------+--------+--------+--------+----* ATATGGCCCGGCCTCTGCCTCCGCAGTGCACGGCTTGGGGCATCTGCTCT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	19	f	2279680	2305799	15	99.9	Perfect prediction

Primer_f	: CTGGTGCTGTTCCTCTACGAC
Primer_r	: TGTACCTGGATGTCAAACCTG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1532

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information