The cDNA fragment was cloned by use of ZAP-cDNA 
synthesis kit and inserted at the EcoRI-XhoI site of 
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with 
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted 
at the EcoRI site of the vector. The 3' end of the cDNA 
was inserted at the XhoI site of the vector.