Vector Information

KIAA0002 sequence was obtained by assembly of 
sequences derived from two clones. ha00029 retains 
sequence from nt. positions 230 to 1821 of KIAA0002. 
The cDNA fragment was cloned by use of ZAP-cDNA 
synthesis kit and inserted at the EcoRI-XhoI site of 
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with 
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted 
at the EcoRI site of the vector. The 3' end of the cDNA 
was inserted at the XhoI site of the vector. The other 
clone a202 harbors sequence from nt. positions -48 to 
396 of KIAA0001 which was amplified by RACE. The 
sequence of -48 to -1 of KIAA0002 which was not
confirmed and therefore not submitted to databases 
are as follows: 148 5' GAGAAGGCTGTGCGTGCTCCTCGCT
TCCTCCGCGGTCTTTCGAGCGGT 3' -1. The RACE product
was cloned into the SmaI site of the M13mp18 vector 
(GenBank Accession No. X02513). Extra 16 residues 
were present between the SmaI site and beginning of 
KIAA0002 sequence.
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