KIAA0002 sequence was obtained by assembly of
sequences derived from two clones. ha00029 retains
sequence from nt. positions 230 to 1821 of KIAA0002.
The cDNA fragment was cloned by use of ZAP-cDNA
synthesis kit and inserted at the EcoRI-XhoI site of
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted
at the EcoRI site of the vector. The 3' end of the cDNA
was inserted at the XhoI site of the vector. The other
clone a202 harbors sequence from nt. positions -48 to
396 of KIAA0001 which was amplified by RACE. The
sequence of -48 to -1 of KIAA0002 which was not
confirmed and therefore not submitted to databases
are as follows: 148 5' GAGAAGGCTGTGCGTGCTCCTCGCT
TCCTCCGCGGTCTTTCGAGCGGT 3' -1. The RACE product
was cloned into the SmaI site of the M13mp18 vector
(GenBank Accession No. X02513). Extra 16 residues
were present between the SmaI site and beginning of
KIAA0002 sequence.
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