KIAA0004 sequence was obtained by assembly of
sequences derived from two clones. ha00065 retains
sequence from nt. positions 381 to 4457 of KIAA0004.
The cDNA fragment was cloned by use of ZAP-cDNA
synthesis kit and inserted at the EcoRI-XhoI site of
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted
at the EcoRI site of the vector. The 3' end of the cDNA
was inserted at the XhoI site of the vector. The 3' end
of the cDNA was inserted at the XhoI site of the vector.
The other clone A65N17 harbors sequence from nt.
positions 1 to 539 of KIAA0004 which was amplified by
RACE. The cDNA fragment of A65N17 was cloned into the
EcoRI-AflII site of pNTT104. Extra 15 residues were
present between the EcoRI site and beginning of