KIAA0004 sequence was obtained by assembly of 
sequences derived from two clones. ha00065 retains 
sequence from nt. positions 381 to 4457 of KIAA0004. 
The cDNA fragment was cloned by use of ZAP-cDNA 
synthesis kit and inserted at the EcoRI-XhoI site of 
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with 
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted 
at the EcoRI site of the vector. The 3' end of the cDNA 
was inserted at the XhoI site of the vector. The 3' end 
of the cDNA was inserted at the XhoI site of the vector. 
The other clone A65N17 harbors sequence from nt. 
positions 1 to 539 of KIAA0004 which was amplified by 
RACE. The cDNA fragment of A65N17 was cloned into the
EcoRI-AflII site of pNTT104. Extra 15 residues were 
present between the EcoRI site and beginning of 
KIAA0004 sequence.